Immunolocalization of a gonadotropin-releasing hormone receptor site in murine endometrium that mediates apoptosis

Cell and Tissue Research
W J Murdoch

Abstract

Circumstantial evidence from a previous study indicated that antibodies generated against a synthetic N-terminal extracellular domain mouse pituitary gonadotropin-releasing hormone (GnRH) receptor peptide acted directly on the murine uterus affecting endometrial regression. Affinity-purified polyclonal sheep antibodies were used to assess tissue-specificity of antibody reactions in diestrous mice. Antibody binding was localized by immunofluorescence staining to anterior pituitary gland and endometrium. Ovary, brain, liver, kidneys, heart, lungs, spleen, gastrointestinal tract, adrenal glands, thymus, thyroid gland, muscle, and adipose were unreactive. Fragmented deoxyribonucleic acid, a marker of programmed cell death/apoptosis, was detected by digoxigenin labeling-immunoperoxidase in endometrial (but not pituitary) glands of animals injected with antipeptide antibodies or native ligand. It appears that luteal phase endometrium of mice expresses a GnRH receptor moiety that is coupled to a cell death (endonuclease) transduction pathway.

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Citations

Aug 26, 2006·Nature Clinical Practice. Endocrinology & Metabolism·Andreas SchäfflerChrista Buechler
Jun 15, 2011·Current Opinion in Obstetrics & Gynecology·Orkun Tan, Orhan Bukulmez
Sep 17, 2009·Reproduction : the Official Journal of the Society for the Study of Fertility·Kirsty Cleverly, T John Wu
Nov 4, 2015·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Francesco M FusiEnrica Capitanio
Jun 21, 2006·Gynecological Endocrinology : the Official Journal of the International Society of Gynecological Endocrinology·Yu-Hung LinChi-Ruey Tzeng
Apr 16, 2008·Fertility and Sterility·Beth W RackowHugh S Taylor
Feb 4, 2009·Journal of Neuroendocrinology·D C SkinnerN Scanlan-Blake
Jan 29, 2008·Experimental Biology and Medicine·Kathryn WaltersT J Wu
Oct 30, 2004·The Journal of Reproduction and Development·Noboru ManabeTakashi Miyano
Apr 25, 2006·Endocrine Reviews·Gaurang S Daftary, Hugh S Taylor
Dec 1, 2019·Reproductive Biology and Endocrinology : RB&E·Francesco M FusiRubens Fadini

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