PMID: 9183747Jun 20, 1997Paper

Immunolocalization of Drosophila eye-specific diacylgylcerol kinase, rdgA, which is essential for the maintenance of the photoreceptor

Journal of Neurobiology
I MasaiY Hotta

Abstract

The Drosophila retinal degeneration A (rdgA) mutant has photoreceptor cells that degenerate within a week after eclosion. The degeneration starts with the disruption of the subrhabdomeric cisternae (SRC), which are the organelles essential for the transport of phospholipids to the photoreceptive membranes. Our previous biochemical and molecular studies suggested that the rdgA gene encodes an eye-specific diacylglycerol kinase (DGK). In this study, we show that retinal degeneration is prevented by the introduction of the eye-DGK gene in the rdgA mutant genome, suggesting that the DGK activity is crucial for the maintenance of the photoreceptor. Furthermore, by immunohistochemical analysis, we have demonstrated that the rdgA protein is predominantly associated with the SRC, suggesting that the conversion from diacylglycerol (DG) to phosphatidic acid (PA) most actively occurs in SRC. The analysis of the eyes of mutants homozygous for rdgA and eye-protein kinase C mutations indicates that retinal degeneration is caused by the deficiency of PA rather than excessive accumulation of DG. From these data, we conclude that the production of PA in the SRC membranes is essential for the maintenance of the photoreceptor.

References

Jul 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·I MasaiY Hotta
Apr 1, 1992·Trends in Biochemical Sciences·V BlankA Israël
May 8, 1992·Science·P HumphriesG J Farrar
Jan 1, 1991·Annual Review of Cell Biology·D P SmithC S Zuker
Feb 1, 1991·Proceedings of the National Academy of Sciences of the United States of America·O BaumannA P Somlyo
Feb 1, 1990·Trends in Biochemical Sciences·H KanohF Sakane
Jan 1, 1990·Proceedings of the National Academy of Sciences of the United States of America·B MinkeZ Selinger
Feb 1, 1989·Journal of Neurocytology·E Matsumoto-SuzukiY Hotta
Apr 1, 1985·Cell·J E O'TousaM L Applebury
May 26, 1987·Nucleic Acids Research·R KlemenzW J Gehring
Nov 4, 1988·Science·G Byrne
Mar 11, 1988·Cell·W H Konigsberg, Y Nemerson
Dec 1, 1974·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·I W McLean, P K Nakane
Nov 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·Y Hotta, S Benzer
Aug 1, 1984·Cell·R E Karess, G M Rubin
Dec 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·S C FujitaS L Shotwell
Aug 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·K Goto, H Kondo
Jan 19, 1995·Nature·J B Hurley
Dec 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·I MasaiY Hotta
Apr 26, 1996·The Journal of Biological Chemistry·M BuntingS M Prescott
Oct 1, 1996·Proceedings of the National Academy of Sciences of the United States of America·K Goto, H Kondo

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Citations

May 10, 2007·Pflügers Archiv : European journal of physiology·Tao Wang, Craig Montell
Aug 7, 2013·Advances in Biological Regulation·Shamshad Cockcroft, Kathryn Garner
Feb 1, 2003·Annual Review of Physiology·Roger C Hardie
Feb 8, 2006·Annual Review of Physiology·Baruch Minke, Moshe Parnas
Mar 21, 2008·Molecular & Cellular Proteomics : MCP·Zhiyin XunDavid E Clemmer
Mar 7, 2014·Journal of Pharmacological Sciences·Mitsue Ishisaka, Hideaki Hara
Oct 30, 2016·Proceedings of the National Academy of Sciences of the United States of America·Mary Anna CarboneTrudy F C Mackay
Mar 4, 2006·Current Biology : CB·Young Kwon, Craig Montell
Jan 21, 2016·Journal of Cell Science·Zuyuan XuDonald F Ready
May 5, 2011·Developmental Neurobiology·Hongai Xia, Donald F Ready
Mar 28, 2002·Physiological Reviews·Baruch Minke, Boaz Cook
Apr 17, 1999·The Journal of Biological Chemistry·M K Topham, S M Prescott
Jun 25, 2019·Frontiers in Cell and Developmental Biology·Rajan ThakurPadinjat Raghu
Nov 26, 1998·The Journal of Biological Chemistry·L DingS M Prescott
Apr 14, 2016·Biochemical Society Transactions·Shweta YadavPadinjat Raghu
Jan 24, 2019·Life Science Alliance·Carina M WeigeltLinda Partridge
May 5, 2001·Biochimica Et Biophysica Acta·M V FrolovJ A Birchler
Jun 8, 2021·Biochimica Et Biophysica Acta. Molecular and Cell Biology of Lipids·Padinjat RaghuHarini Krishnan
Mar 21, 2006·Molecular Genetics and Metabolism·Julian A Martinez Agosto, Edward R B McCabe

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