Immunolocalization of myosin Va in the developing nervous system of embryonic chicks

Anatomy and Embryology
Alexandre AzevedoRoy E Larson

Abstract

Myosins are molecular motors associated with the actin cytoskeleton that participate in the mechanisms of cellular motility. During the development of the nervous system, migration of nerve cells to specific sites, extension of growth cones, and axonal transport are dramatic manifestations of cellular motility. We demonstrate, via immunoblots, the expression of myosin Va during early stages of embryonic development in chicks, extending from the blastocyst period to the beginning of the fetal period. The expression of myosin Va in specific regions and cellular structures of the nervous system during these early stages was determined by immunocytochemistry using a polyclonal antibody. Whole mounts of chick embryos at 24-30-h stages showed intense immunoreactivity of the neural tube in formation along its full extent. Cross-sections at these stages of development showed strong labeling in neuroepithelial cells at the basal and apical regions of the neural tube wall. Embryos at more advanced periods of development (48 h and 72 h) showed distinctive immunolabeling of neuroepithelial cells, neuroblasts and their cytoplasmic extensions in the mantle layer of the stratified neural tube wall, and neuroblasts and their cytoplasmic extens...Continue Reading

Citations

Apr 6, 2013·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·C C R SouzaA R Martins
Feb 11, 2011·Genesis : the Journal of Genetics and Development·Ricardo Moraes BorgesChao Yun Irene Yan
Dec 5, 2013·Cell Biology International·Tatiane Y N KannoChao Yun Irene Yan
Aug 24, 2010·Brazilian Journal of Medical and Biological Research = Revista Brasileira De Pesquisas Médicas E Biológicas·L S BrinnA R Martins
Sep 8, 2010·Biochemical and Biophysical Research Communications·Hiroaki KubotaShin'ichi Ishiwata

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