Immunolocalization of Pit-1 in gonadotroph nuclei is indicative of the transdifferentiation of gonadotroph to lactotroph cells in prolactinomas induced by estrogen

Histochemistry and Cell Biology
Jorge Humberto MukdsiAlicia Inés Torres

Abstract

The pituitary protein transcription factor (Pit-1) regulates the differentiation and proliferation of somatotrophs, lactotrophs, and thyrotrophs and the c-Myc oncoprotein plays a critical role in somatotroph and lactotroph differentiation. Both were involved in the genesis of pituitary tumors. The combined analysis of Pit-1 and c-Myc expression and the morphometric and biochemical parameters of the lactotroph population after treatment with estrogen for 7, 20, and 60 days provided new information on molecular mechanisms implicated in the formation of prolactinomas. Estrogen treatment for 7 days caused a significant proliferation of lactotrophs (70%) and this increase reached an additional 55% at 60 days. The proliferation of lactotrophs was concurrent with higher serum and pituitary prolactin levels. An augmentation of Pit-1 and c-Myc expression in both cytoplasmic and nuclear extracts after estrogen can be associated with lactotroph proliferation. Moreover, the multistep correlation analysis revealed that the expression of nuclear Pit-1 was the strongest predictor of prolactinoma development. Also the Pit-1 immunolocalization in nuclei of gonadotrophs suggests the activation of genes involved in transdifferentiation of gonadot...Continue Reading

Citations

Dec 23, 2004·Histochemistry and Cell Biology·Esther Asan, Detlev Drenckhahn
Sep 1, 2005·Histochemistry and Cell Biology·Silvina GutiérrezElsa Margarita Orgnero
Feb 27, 2008·Histochemistry and Cell Biology·Ana Lucía De PaulAlicia Inés Torres
Sep 1, 2005·Cell and Tissue Research·Shunsuke MiyaiRobert Yoshiyuki Osamura
May 31, 2006·Journal of Molecular Histology·Jorge Humberto MukdsiAlicia Inés Torres
Jan 23, 2014·Mammalian Genome : Official Journal of the International Mammalian Genome Society·Scott G KurzJames D Shull
Jul 15, 2009·Toxicology and Applied Pharmacology·Claudia Mariela PalmeriAlicia Inés Torres
Jun 7, 2008·Molecular and Cellular Endocrinology·Juan Pablo PetitiAlicia Inés Torres
Feb 14, 2006·Molecular and Cellular Endocrinology·Twila A JacksonAndrew P Bradford
Aug 6, 2013·Journal of Toxicologic Pathology·Hironobu YasunoRyo Fukuda
Mar 21, 2015·Endocrine-related Cancer·Maria Eugenia SabatinoAna Lucía De Paul
Oct 26, 2005·Experimental Biology and Medicine·Allison M BenoitCharles A Blake
May 9, 2013·American Journal of Physiology. Endocrinology and Metabolism·Liliana del V SosaAlicia I Torres

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