Immunolocalization of the Anti-Müllerian Hormone (AMH) in Caprine Follicles and the Effects of AMH on In Vitro Culture of Caprine Pre-antral Follicles Enclosed in Ovarian Tissue

Reproduction in Domestic Animals = Zuchthygiene
Rebeca M P RochaJ R Figueiredo

Abstract

The aims of this study were to evaluate the localization, by immunohistochemistry, of the anti-Müllerian hormone (AMH) in goat ovaries and to investigate its effects on the in vitro survival and development of caprine pre-antral follicles enclosed in fragments of ovarian tissue. Pre-antral follicles were cultured in vitro for 1 or 7 days in α-MEM(+) in the absence or presence of kit ligand (KL; 50 ng/ml, positive control) or AMH (50 or 150 ng/ml). The results showed that AMH was localized in oocytes and granulosa cells from the primordial follicle to antral follicle stages. Addition of AMH maintained the percentage of developing follicles, similar to that in the uncultured control; however, the percentage of developing follicles was significantly lower than that in the cultured control and KL. Nonetheless, addition of AMH to the culture medium did not affect survival rates and follicular growth. In conclusion, this study demonstrated that the expression of AMH varies according to the compartment and stage of follicular development. Furthermore, AMH inhibits the activation of caprine primordial follicles.

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Citations

Jan 29, 2017·The Journal of Endocrinology·Michael W Pankhurst
Mar 16, 2018·The Journal of Endocrinology·Michael W PankhurstNicola J Batchelor
Jun 23, 2018·Biology of Reproduction·R A LemckeP A Johnson
Mar 14, 2018·Reproduction, Fertility, and Development·Onalenna KereilweHiroya Kadokawa
Oct 19, 2019·Animals : an Open Access Journal From MDPI·Shuang YangJiwen Wang
Mar 26, 2021·Frontiers in Veterinary Science·Devika GautamSachinandan De

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