Immunolocalization of the human basal epithelial marker monoclonal antibody 312C8-1 in normal tissue and mammary tumours of rodents.

Virchows Archiv. A, Pathological Anatomy and Histopathology
A TsuburaS H Dairkee

Abstract

Using immunoperoxidase staining of monoclonal antibody 312C8-1 against 51,000 dalton human keratin polypeptide, immunolocalization was observed in frozen sections of normal tissue and mammary tumours of adult female mice and rats. In normal tissue, the epitope was recognized in myoepithelial cells of the mammary, sweat and salivary glands, and in basal and suprabasal cells of the epidermis. However, the antibody did not react with luminal epithelial cells of the above glands or with mesenchymal cells. In spontaneous mammary tumours of mice, marker-positive tumour cells were distributed only in the outer layer of adenocarcinoma Type A, while they were scattered in some foci of adenocarcinoma Type B, and encircled the epithelial foci of pregnancy dependent tumours (plaque). All layers of epidermoid structures in adenoacanthoma revealed positivity. In rat mammary tumours induced by local dusting with 7, 12-dimethylbenz(a)anthracene (DMBA) powder, the staining pattern of benign tumours was comparable to that of the normal mammary gland. But, in addition to basally situated cells, marker-positive tumour cells were found scattered in the foci of adenocarcinoma, and were not restricted to basal cells in squamous cell carcinoma. The ma...Continue Reading

References

Sep 1, 1979·The Journal of Pathology·J V Forrester, I Grierson
Nov 1, 1985·Proceedings of the National Academy of Sciences of the United States of America·S H DairkeeA J Hackett
Jul 1, 1986·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·R B NagleE D Jarasch
Oct 1, 1987·Breast Cancer Research and Treatment·S H DairkeeA Hackett
Jul 6, 1988·Journal of the National Cancer Institute·S H DairkeeA J Hackett
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·A TsuburaA Matsuzawa
Jan 1, 1988·Virchows Archiv. A, Pathological Anatomy and Histopathology·A TsuburaS Morii
Jan 1, 1971·Calcified Tissue Research·R L Speirs
Mar 1, 1980·The Journal of Cell Biology·W W FrankeT W Keenan
Sep 1, 1981·Proceedings of the National Academy of Sciences of the United States of America·B B AschT T Sun
Feb 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·R AllenB Armstrong

❮ Previous
Next ❯

Related Concepts

Related Feeds

Carcinoma, Squamous Cell

Basal cell carcinoma is a form of malignant skin cancer found on the head and neck regions and has low rates of metastasis. Discover the latest research on basal cell carcinoma here.