PMID: 7538665May 9, 1995Paper

Immunolocalization of the mercurial-insensitive water channel and glycerol intrinsic protein in epithelial cell plasma membranes

Proceedings of the National Academy of Sciences of the United States of America
A FrigeriA S Verkman

Abstract

Two water channel homologs were cloned recently from rat kidney, mercurial-insensitive water channel (MIWC) and glycerol intrinsic protein (GLIP). Polyclonal antibodies were raised against synthetic C-terminal peptides and purified by affinity chromatography. MIWC and GLIP antibodies recognized proteins in rat kidney with an apparent molecular mass of 30 and 27 kDa, respectively, and did not cross-react. By immunofluorescence, MIWC and GLIP were expressed together on the basolateral plasma membrane of collecting duct principal cells in kidney. By immunohistochemistry, MIWC and GLIP were expressed on tracheal epithelial cells with greater expression of GLIP on the basal plasma membrane and MIWC on the lateral membrane; only MIWC was expressed in bronchial epithelia. In eye, GLIP was expressed in conjunctival epithelium, whereas MIWC was found in iris, ciliary body, and neural cell layers in retina. MIWC and GLIP colocalized on the basolateral membrane of villus epithelial cells in colon and brain ependymal cells. Expression of MIWC and GLIP was not detected in small intestine, liver, spleen, endothelia, and cells that express water channels CHIP28 or WCH-CD. These studies suggest water/solute transporting roles for MIWC and GLIP...Continue Reading

References

May 1, 1991·Trends in Biochemical Sciences·G J WistowA B Chepelinsky
Jan 1, 1987·The Journal of Membrane Biology·K Strange, K R Spring
Dec 15, 1993·Biochemical and Biophysical Research Communications·T MaA S Verkman
May 24, 1994·Proceedings of the National Academy of Sciences of the United States of America·H G FolkessonA S Verkman
Dec 15, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenH W Harris
Aug 1, 1993·Proceedings of the National Academy of Sciences of the United States of America·S NielsenP Agre

❮ Previous
Next ❯

Citations

Jan 13, 2009·Inflammatory Bowel Diseases·Jonathan J HansenR Balfour Sartor
Feb 7, 2001·Biochemical and Biophysical Research Communications·Z VajdaS Nielsen
Oct 12, 2000·Biochemical and Biophysical Research Communications·L N NejsumS Nielsen
Jul 1, 1995·Histochemistry and Cell Biology·D BrownI Sabolic
Jul 27, 2006·The Journal of Membrane Biology·M H Levin, A S Verkman
Nov 28, 2012·The Journal of Membrane Biology·Miwako Matsuki-FukushimaHiroshi Sugiya
Sep 1, 2005·Acta Neuropathologica·Kazuko Aoki-YoshinoYoshihiro Wakayama
Jun 21, 2008·Histochemistry and Cell Biology·Kuniaki TakataTakahiro Hasegawa
Jul 13, 2004·Pflügers Archiv : European journal of physiology·Kimiya SatoSeiichi Tamai
Apr 14, 2010·Journal of Molecular Neuroscience : MN·Meng-Ling ChenZhi-Qi Zhao
Jul 9, 2013·Brain Research·Rui GeXiao-chuan Xiong
Mar 11, 2004·Brain Research. Molecular Brain Research·Masakazu MiyajimaKiyoshi Sato
Feb 5, 2003·Experimental Eye Research·A S Verkman
Jun 29, 1999·Journal of the Neurological Sciences·J W LiuH Oniki
Feb 13, 2001·Respiration Physiology·C A HalesD A Quinn
Dec 14, 2001·Journal of Neuroscience Methods·E I SolenovA S Verkman
Apr 20, 2001·Brain Research. Molecular Brain Research·K YonedaT Kato
Dec 15, 2000·Progress in Neurobiology·J L VeneroJ Cano
Jan 4, 2001·Neuroscience Letters·H KobayashiA Wada
May 13, 1998·Neuroscience Letters·I B KimM H Chun
May 10, 2011·Acta Pharmacologica Sinica·Alan S VerkmanLukmanee Tradtrantip
Nov 7, 2002·Anatomical Science International·Toshiyuki MatsuzakiKuniaki Takata
Jan 25, 2003·Journal of Gastroenterology and Hepatology·Akihiko ItohTadao Bamba
Jun 11, 2003·Journal of Gastroenterology and Hepatology·Tomoyuki TsujikawaYoshihide Fujiyama
Aug 7, 2002·Journal of Anatomy·A S Verkman
Oct 10, 1998·Kidney International·T Yamamoto, S Sasaki
Oct 15, 2003·The Journal of General Physiology·Yuanlin SongA S Verkman
Nov 20, 1998·The American Journal of the Medical Sciences·P M Deen, N V Knoers
Nov 20, 1998·The American Journal of the Medical Sciences·A S Verkman
Dec 25, 2004·Neuroreport·Solveig TenckhoffAndreas Bringmann
Oct 27, 2007·Annual Review of Physiology·Aleksandra RojekRobert A Fenton
Mar 20, 2009·American Journal of Physiology. Renal Physiology·Alfred N Van HoekRajkumar V Patil

❮ Previous
Next ❯

Related Concepts

Related Feeds

CSF & Lymphatic System

This feed focuses on Cerebral Spinal Fluid (CSF) and the lymphatic system. Discover the latest papers using imaging techniques to track CSF outflow into the lymphatic system in animal models.