Jan 1, 1978

Immunologic and nonimmunologic generation of superoxide from mast cells and basophils

The Journal of Clinical Investigation
W R Henderson, M Kaliner


Mediator release from rat peritoneal and human lung mast cells as well as human leukemic basophils was examined to determine whether super-oxide (O(-) (2)) was concomitantly generated. Immunologic or nonimmunologic stimulation of each preparation induced parallel release of histamine and O(-) (2) within 2 min. O(-) (2) production was quantitated by superoxide dismutase (SOD)-inhibitable chemiluminescence and cytochrome c reduction. SOD was detected in basophil and mast cell lysates and was also released by rat mast cells stimulated by anti-IgE. Secretory granules isolated from purified rat mast cells released histamine, O(-) (2), and SOD upon exposure to cations. Thus, both superoxide radicals and SOD may play a role in host defenses involved in immediate hypersensitivity reactions.

Mentioned in this Paper

Mast Cell
Immediate Hypersensitivity
Energy Metabolism
Hairy Cell Leukemia
Ag-Zn Superoxide Dismutase
Histamine Release
Cellular Immune Response

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