PMID: 1104747Dec 1, 1975

Immunologic properties of bacterial lipopolysaccharide (LPS). II. The unresponsiveness of C3H/HeJ Mouse spleen cells to LPS-induced mitogenesis is dependent on the method used to extract LPS

The Journal of Experimental Medicine
B J SkidmoreW O Weigle

Abstract

The C3H/HeJ mouse strain, previously shown to be a nonresponder to bacterial lipopolysaccharide (LPS)-induced mitogenesis in vitro, was demonstrated by the present studies to be competent to respond mitogenically to LPS, but only to LPS preparations obtained by selected extraction methods. These preparations appear to be confined to LPS isolated by mild extraction techniques, such as TCA or butanol. In contrast, those obtained by techniques utilizing phenol were only weakly stimulatory or completely nonstimulatory for spleen cells from the C3H/HeJ. All LPS preparations tested, on the other hand, were highly stimulatory for cells from another mouse strain, namely the C3H/St. The critical importance of the method of extraction of LPS on its mitogenic activity for C3H/HeJ cells was stressed by experiments in which LPS was prepared from Escherichia coli K235 using either of two procedures. In these experiments, phenol-extracted LPS, although mitogenic in the C3H/St, was completely nonstimulatory in the C3H/HeJ; whereas, butanol-extracted LPS was highly stimulatory in both strains of mice. This striking difference was attributed to a destructive effect of phenol on LPS, as demonstrated by the fact that treatment of butanol LPS with ...Continue Reading

References

Jun 1, 1975·The Journal of Experimental Medicine·M D Jacobs, D C Morrison
Aug 1, 1972·Cellular Immunology·J AnderssonO Sjöberg
Jul 1, 1973·Proceedings of the National Academy of Sciences of the United States of America·J M ChillerW O Weigle
Oct 1, 1973·European Journal of Immunology·M H JuliusL A Herzenberg
Feb 1, 1974·The Journal of Experimental Medicine·M F KagnoffM Cohn
Jan 1, 1974·Scandinavian Journal of Immunology·E GronowiczG Möller
Nov 1, 1974·European Journal of Immunology·E Gronowicz, A Coutinho
Mar 1, 1971·European Journal of Biochemistry·C GalanosO WESTPHAL
Jun 30, 1966·Annals of the New York Academy of Sciences·A NowotnyD J Tripodi
Jul 13, 1972·European Journal of Biochemistry·E T RietschelO WESTPHAL
Jun 30, 1966·Annals of the New York Academy of Sciences·O LUDERITZJ Schlosshardt
Jun 1, 1969·European Journal of Biochemistry·C GalanosO WESTPHAL
Sep 1, 1967·The Journal of Experimental Medicine·R I Mishell, R W Dutton
Jul 15, 1958·Proceedings of the National Academy of Sciences of the United States of America·M Meselson, F W Stahl

Citations

May 1, 1995·Inflammation Research : Official Journal of the European Histamine Research Society ... [et Al.]·B Henderson, M Wilson
May 10, 1979·Journal of Immunological Methods·D C Morrison, B J Curry
Mar 1, 1979·Immunopharmacology·B J Curry, D C Morrison
Aug 1, 1992·Immunology Today·J A Majde
Sep 1, 1976·The Journal of Experimental Medicine·D C MorrisonD M Jacobs
Feb 1, 1977·The Journal of Experimental Medicine·P W Kincade
Oct 1, 1978·The Journal of Experimental Medicine·G P HaasA Nowotny
Jan 1, 1977·Scandinavian Journal of Immunology·O TurunenA de la Chapelle
Jan 28, 1998·Journal of Periodontology·H YamaguchiJ Nakamura
Jan 1, 1975·Transplantation Reviews·E Gronowicz, A Coutinho
Jan 1, 1975·Transplantation Reviews·G Möller
Jan 1, 1975·Transplantation Reviews·J Watson
Jan 30, 2013·PloS One·Sanhita RoyAlan M Tartakoff
Aug 25, 2001·Journal of Endotoxin Research·S N Vogel
Jul 24, 2004·American Journal of Physiology. Regulatory, Integrative and Comparative Physiology·C von MeyenburgW Langhans
Jul 6, 2000·The Journal of Immunology : Official Journal of the American Association of Immunologists·M HirschfeldJ J Weis
Nov 1, 1977·CRC Critical Reviews in Toxicology·L J Berry

Related Concepts

DNA, Double-Stranded
Alkalescens-Dispar Group
Lipopolysaccharides
Lymphoid Cells
Mice, Inbred Strains
Phytomitogens
Polysaccharides, Bacterial
Spleen

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Systemic Juvenile Idiopathic Arthritis

Systemic juvenile idiopathic arthritis is a rare rheumatic disease that affects children. Symptoms include joint pain, but also fevers and skin rashes. Here is the latest on this disease.

Chromatin Regulation and Circadian Clocks

The circadian clock plays an important role in regulating transcriptional dynamics through changes in chromatin folding and remodelling. Discover the latest research on Chromatin Regulation and Circadian Clocks here.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Myocardial Stunning

Myocardial stunning is a mechanical dysfunction that persists after reperfusion of previously ischemic tissue in the absence of irreversible damage including myocardial necrosis. Here is the latest research.

Pontocerebellar Hypoplasia

Pontocerebellar hypoplasias are a group of neurodegenerative autosomal recessive disorders with prenatal onset, atrophy or hypoplasia of the cerebellum, hypoplasia of the ventral pons, microcephaly, variable neocortical atrophy and severe mental and motor impairments. Here is the latest research on pontocerebellar hypoplasia.

Cell Atlas Along the Gut-Brain Axis

Profiling cells along the gut-brain axis at the single cell level will provide unique information for each cell type, a three-dimensional map of how cell types work together to form tissues, and insights into how changes in the map underlie health and disease of the GI system and its crosstalk with the brain. Disocver the latest research on single cell analysis of the gut-brain axis here.

Chronic Traumatic Encephalopathy

Chronic Traumatic Encephalopathy (CTE) is a progressive degenerative disease that occurs in individuals that suffer repetitive brain trauma. Discover the latest research on traumatic encephalopathy here.