Immunological analysis of porin polymorphism in Escherichia coli B and K-12.

Molecular Immunology
N LupiJ M Pagès

Abstract

Two sets of monoclonal antibodies (MoF type I and MoF type II) directed against the OmpF protein were used to analyze the immunological reactivity of the major outer membrane porins of E. coli B and K-12. All these antibodies present a specificity to the native OmpF protein. In addition, among the type II antibodies, MoF 18, 19 and 20 could recognize an epitope present on both monomeric and trimeric forms of the porin as demonstrated by immunoblotting analyses. The use of two different screening methods led to the isolation of two different sets of MoF, one specific for a native conformation accessible only on E. coli B strain and the second directed against epitopes present on OmpF of the two strains, B and K-12. These various responses are discussed in relation to the lipopolysaccharide binding to OmpF and with respect to the screening test used.

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Citations

Feb 1, 1996·FEMS Microbiology Letters·V SimonetJ M Pages
Nov 25, 1998·Research in Microbiology·R el KouhenJ M Pagès
Oct 1, 1990·Molecular Microbiology·J P BourdineaudJ M Pagès
May 15, 1992·European Journal of Biochemistry·D FourelJ M Pagès
Jun 15, 1993·European Journal of Biochemistry·R el KouhenJ M Pagès
Sep 1, 1994·European Journal of Biochemistry·R el KouhenJ M Pagès
Jan 20, 2000·Antimicrobial Agents and Chemotherapy·V SimonetJ M Pagès
Sep 10, 2008·BMC Microbiology·Matthew A Lambert, Stephen G J Smith
Dec 1, 1995·Infection and Immunity·S P SinghT Abdullah

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