Immunological and biochemical characterizations of coxsackievirus A6 and A10 viral particles

Antiviral Research
Chia-Chyi LiuPele Chong

Abstract

Childhood exanthema caused by different serotypes of coxsackievirus (CV-A) and enterovirus A71 (EV-A71) has become a serious global health problem; it is commonly known as hand, foot, and mouth disease (HFMD). Current EV-A71 vaccine clinical trials have demonstrated that human antibody responses generated by EV-A71 vaccinations do not cross-neutralize coxsackievirus A16 (CV-A16). An effective multivalent HFMD vaccine is urgently needed. From molecular epidemiological studies in Southeast Asia, CV-A6 and CV-A10 are commonly found in HFMD outbreaks. In this study, CV-A6 and CV-A10 were individually cultured in rhabdomyosarcoma (RD) cells grown in medium containing serum, harvested and concentrated. In viral downstream purification, two viral fractions were separated by sucrose gradient zonal ultracentrifugation and detected using a SDS-PAGE analysis and a virus infectivity assay. These two viral fractions were formalin-inactivated, and only the infectious particle fraction was found to be capable of inducing CV-A serotype-specific neutralizing antibody responses in animal immunogenicity studies. These mouse and rabbit antisera also failed to cross-neutralize EV-A71 and CV-A16 infections. Only a combination of formalin-inactivated...Continue Reading

References

Oct 16, 2007·Journal of Medical Virology·Fan-Chen TsengUNKNOWN CDC-Taiwan Virology Reference Laboratories and Sentinel Physician Network
Apr 29, 2011·Journal of Microbiology, Immunology, and Infection = Wei Mian Yu Gan Ran Za Zhi·Shih-Hsuan LoTzou-Yien Lin
Feb 7, 2012·Emerging Infectious Diseases·Tsuguto FujimotoHiroyuki Shimizu
Mar 13, 2012·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·A MirandH Peigue-Lafeuille
Sep 7, 2012·PLoS Neglected Tropical Diseases·Min-Shi LeeIh-Jen Su
May 3, 2013·Journal of Virology·Javier O CifuenteSusan Hafenstein
Sep 17, 2013·Clinical Microbiology and Infection : the Official Publication of the European Society of Clinical Microbiology and Infectious Diseases·M CabrerizoG Trallero
Nov 16, 2013·Human Vaccines & Immunotherapeutics·Qunying MaoZhenglun Liang
Jan 25, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Eli Ben-ChetritDana G Wolf
Feb 28, 2014·The New England Journal of Medicine·Fengcai ZhuNan Wang
Apr 5, 2014·Euro Surveillance : Bulletin Européen Sur Les Maladies Transmissibles = European Communicable Disease Bulletin·C SinclairH Harvala
Apr 11, 2014·Archives of Virology·Magilé C FonsecaGuelsys González
Jun 17, 2014·Journal of Clinical Virology : the Official Publication of the Pan American Society for Clinical Virology·Marigdalia K Ramirez-FortStephen K Tyring
Aug 16, 2014·Journal of Paediatrics and Child Health·Rebecca HaymanEmma Best
Sep 16, 2014·The Pediatric Infectious Disease Journal·Jin Ho Chong, Mark Koh Jean Aan
Sep 23, 2014·International Journal of Infectious Diseases : IJID : Official Publication of the International Society for Infectious Diseases·Jing-Fang ChenBian-Cheng Sun
Oct 30, 2014·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Pele ChongMichel Klein

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Citations

Feb 23, 2019·Expert Review of Anti-infective Therapy·Lianlian BianZhenglun Liang
Dec 5, 2019·Therapeutic Advances in Vaccines and Immunotherapy·Hui Xuan Lim, Chit Laa Poh
Sep 13, 2017·Nature Communications·Longfa XuNingshao Xia
Sep 19, 2018·Emerging Infectious Diseases·Yu LiBenjamin J Cowling
Mar 20, 2021·Emerging Microbes & Infections·Sha-Sha QianShuo Shen

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