Abstract
Dermonecrotic toxin (DNT) produced by Bordetella bronchiseptica (B. bronchiseptica) can cause clinical turbinate atrophy in swine and induce dermonecrotic lesions in model mice. We know that the N-terminal of DNT molecule contains the receptor-binding domain, which facilitates binding to the target cells. However, we do not know whether this domain has sufficient immunogenicity to resist B. bronchiseptica damage and thereby to develop a subunit vaccine for the swine industry. In this study, we prokaryotically expressed the recombinant N-terminal of DNT from B. bronchiseptica (named DNT-N) and prepared it for the subunit vaccine to evaluate its immunogenicity. Taishan Pinus massoniana pollen polysaccharide (TPPPS), a known immunomodulator, was used as the adjuvant to examine its immune-conditioning effects. At 49 d after inoculation, 10 mice from each group were challenged with B. bronchiseptica, and another 10 mice were intradermally challenged with native DNT, to examine the protection imparted by the vaccines. The immune parameters (T-lymphocyte counts, cytokine secretions, serum antibody titers, and survival rates) and skin lesions were determined. The results showed that pure DNT-N vaccine significantly induced immune respo...Continue Reading
References
Oct 1, 1988·Veterinary Microbiology·T MagyarG A Hall
Feb 1, 1971·Journal of Medical Microbiology·T Iida, T Okonogi
Nov 1, 1993·Archives of Surgery·S F Lowry
Aug 1, 1997·Clinical Biochemistry·S LiuG Jackowski
Jan 18, 2002·Infection and Immunity·Susan L BrockmeierRobert A Kunkle
Mar 13, 2003·Developmental and Comparative Immunology·Takuya IrieHiroshi Kodama
Mar 15, 2006·European Journal of Pharmaceutics and Biopharmaceutics : Official Journal of Arbeitsgemeinschaft Für Pharmazeutische Verfahrenstechnik E.V·Mi Lan KangHan Sang Yoo
Feb 24, 2007·Microbes and Infection·Lakshmi GopinathanEric T Harvill
May 15, 2010·Current Opinion in Immunology·M Lamine MbowRino Rappuoli
Jul 21, 2010·Nature Immunology·Andrea C Carpenter, Rémy Bosselut
Feb 8, 2011·Vaccine·Kai WeiRuiliang Zhu
Feb 26, 2013·Cellular Immunology·Yunpeng FanYuanliang Hu
Jun 19, 2013·Carbohydrate Polymers·Yee HuangDeyun Wang
Oct 1, 2013·International Immunopharmacology·Xue ZhaoRuiLiang Zhu
Feb 11, 2014·Research in Veterinary Science·Fanxia GuoRuiliang Zhu
Citations
Feb 2, 2016·Molecular and Cellular Probes·Jun PengRuiliang Zhu
Nov 7, 2019·Vaccines·Weicheng AiBin Wu
Sep 18, 2020·BMC Veterinary Research·Yu TaoYulong He
Jun 21, 2017·Allergologia et immunopathologia·J ZhouR Zhu
Jun 18, 2018·Vaccine·Jiansong ZhangYufeng Li