Immunological and proteomic analysis of preparative isoelectric focusing separated culture filtrate antigens of Mycobacterium tuberculosis

Experimental and Molecular Pathology
Anbarasu DeenadayalanAlamelu Raja

Abstract

Isolation of the secreted proteins and studying the immune response they induce is an essential prerequisite for understanding the pathogenesis of M. tuberculosis. In this study, preparative liquid-phase isoelectric focusing was used for the separation of culture filtrate protein (CFP) of M. tuberculosis. This procedure resolved culture filtrate proteins into 20 fractions with a pI range of 2.59 to 12.9. These 20 fractions were subjected to immunological analysis in healthy laboratory volunteers from our endemic area. Eleven fractions (Fractions 5, 6, 7, 8, 9, 10, 11, 13, 15, 16, and 19) showed increased interferon gamma (IFN-gamma) secretion and 5 fractions induced increased proliferative response, when compared to unfractionated CFP. In the 11 fractions which showed increased IFN-gamma secretion, mass spectrometric analysis identified 19 different proteins. Apart from the already reported immunodominant antigens like FbpB, CFP-10 and ESAT-6, two new T cell antigens (AcpM and PpiA) were also identified in the immunologically active fractions. Immunoinformatic analysis showed that PpiA was predicted to bind more number of class I and class II HLA alleles compared with the immunodominant ESAT-6 and CFP-10. Population coverage ca...Continue Reading

References

Jan 1, 1995·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·P E Fine
Dec 26, 2001·Bioinformatics·H Singh, G P Raghava
Apr 23, 2004·Infection and Immunity·Alberto Diaz-QuiñonezVianney Ortiz-Navarrete
Jan 20, 2005·Electrophoresis·Pier Giorgio RighettiEgisto Boschetti
Jul 14, 2005·Nature Reviews. Microbiology·Peter Andersen, T Mark Doherty
Oct 29, 2005·Tuberculosis·Rohini QamraBrian Henderson
Aug 12, 2006·Expert Review of Proteomics·Sunny Tam
Oct 21, 2006·Science·R Andres FlotoPaul J Lehner
Feb 26, 2008·American Journal of Respiratory and Critical Care Medicine·Wing Wai Yew, Chi Chiu Leung

❮ Previous
Next ❯

Related Concepts

Related Feeds

Bioinformatics in Biomedicine

Bioinformatics in biomedicine incorporates computer science, biology, chemistry, medicine, mathematics and statistics. Discover the latest research on bioinformatics in biomedicine here.