PMID: 9429211Jan 1, 1997Paper

Immunological approach to hepatocellular carcinoma

Journal of Viral Hepatitis
Jack R WandsS Tanaka

Abstract

A library of monoclonal antibodies (MoAbs) has been produced against a human hepatocellular carcinoma (HCC) cell line designated FOCUS in order to study the antigenic properties of transformed hepatocytes. Several monoclonal antibodies (MoAbs) were initially selected for study since they bound to antigens which were overexpressed in HCC tissues compared with the adjacent uninvolved normal liver counterpart; in addition, these MoAbs revealed low level antigen expression on other normal human tissues. Subsequently, HCC cell lines were metabolically labelled and the antigens further characterized by immunoprecipitation and Western blot analysis. If the MoAb recognized a primary linear epitope on a protein, cloning was performed using a lambda GT11 cDNA expression library prepared from the FOCUS HCC cell line. These studies characterized the HCC associated antigen(s) at the molecular level. This review illustrates the value of such an experimental approach to search for and identify HCC associated antigens and emphasizes the biological properties of novel proteins may be defined and characterized by these techniques. More important, our investigations have described unique proteins that may not only be important in the pathogenesis...Continue Reading

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Citations

Jan 26, 2008·Advances in Medical Sciences·H E Blum
Jul 28, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·M Chiara CantariniJack R Wands
Apr 22, 2006·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Mark Branda, Jack R Wands
Jun 26, 2010·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Kui WangMeng-Chao Wu
Nov 16, 2004·Cancer Detection and Prevention·Takashi MaedaSuzanne M de la Monte
Oct 6, 2006·Human Pathology·JiYi WangMurray B Resnick
Mar 28, 2006·Journal of Hepatology·Suzanne M de la MonteJack R Wands

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