Immunological assessment of the distribution of type VII adenylyl cyclase in brain

Brain Research
N MonsB Tabakoff

Abstract

The localization of the nine identified isoforms of adenylyl cyclase in brain has been largely based on determination of patterns of mRNA expression. A polyclonal antibody has now been developed that specifically recognizes Type VII adenylyl cyclase. This antibody was used for immunocytochemical analysis of the distribution of Type VII adenylyl cyclase in rat brain. Labeling of Type VII adenylyl cyclase was observed in several areas, including cerebellum, caudate-putamen, nucleus accumbens, hippocampus and cerebral cortex. In some of these areas, the staining of the adenylyl cyclase protein suggested the possibility of presynaptic localization. For example, in situ hybridization showed Type VII adenylyl cyclase mRNA concentrated in cerebellar granule neurons. The cerebellar granule cell layer, however, showed little immunostaining, while punctate immunostaining was observed in the molecular layer. These results suggested that protein synthesized in the granule neurons may be targeted to the neuron terminals. Punctate staining in the caudate-putamen, globus pallidus and nucleus accumbens also suggested the possibility of axonal and/or dendritic localization of Type VII adenylyl cyclase in these regions. Labeling of the soma of c...Continue Reading

References

Aug 1, 1992·Proceedings of the National Academy of Sciences of the United States of America·M Yoshimura, D M Cooper
May 1, 1992·Trends in Pharmacological Sciences·G F Koob
Nov 15, 1991·Proceedings of the National Academy of Sciences of the United States of America·P G FeinsteinR R Reed
Aug 29, 1995·Proceedings of the National Academy of Sciences of the United States of America·N MonsD M Cooper
May 12, 1995·The Journal of Biological Chemistry·K HellevuoB Tabakoff
May 26, 1995·The Journal of Biological Chemistry·G IwamiY Ishikawa
Oct 25, 1994·Proceedings of the National Academy of Sciences of the United States of America·O Jacobowitz, R Iyengar
Apr 15, 1993·Biochemical and Biophysical Research Communications·K HellevuoB Tabakoff
Jan 1, 1996·Annual Review of Pharmacology and Toxicology·R K SunaharaA G Gilman
Dec 1, 1995·Alcoholism, Clinical and Experimental Research·M Yoshimura, B Tabakoff

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Citations

Mar 17, 2005·Alcoholism, Clinical and Experimental Research·Timothy DonohueBoris Tabakoff
Oct 28, 2006·Alcoholism, Clinical and Experimental Research·Masami YoshimuraCristina E Gonzalez
Oct 6, 2005·Alcoholism, Clinical and Experimental Research·Douglas B MatthewsRobert W Williams
Mar 27, 2001·Annual Review of Pharmacology and Toxicology·J Hanoune, N Defer
Mar 3, 2004·Annals of the New York Academy of Sciences·Martin J KellyOline K Rønnekleiv
Jun 8, 2001·Alcoholism, Clinical and Experimental Research·P L HoffmanD S Choi
Mar 30, 2006·Neurochemical Research·Ferenc A AntoniJames Simpson
May 3, 2021·Movement Disorders : Official Journal of the Movement Disorder Society·Arianna FerriniManju A Kurian
Apr 14, 2000·Brain Research. Molecular Brain Research·M J DrescherD G Drescher
Aug 17, 2005·Pharmacology & Therapeutics·P M Newton, R O Messing

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