Immunological characterization and fine localization of a lizard beta-keratin

Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution
L Alibardi, M Toni

Abstract

Scales of lizards contain beta-keratin of poorly known composition. In the present study, a rat polyclonal serum against a lizard beta-keratin of 14-15 kDa has been produced and the relative protein has been immunolocalized in the epidermis. The observations for the first time show that the isolated protein band derives from the extraction of a protein component of the beta-keratin filaments of lizard epidermis. In immunoblots and immunocytochemistry, the antiserum recognizes most lizard beta-keratins, but produces a variable cross-reactivity with snake beta-keratins, and weak or no reactivity with beta-keratins isolated from tuatara, turtles, alligator and birds. In bidimensional immunoblots of lizard epidermis, three main spots at 15-16 kDa with isoelectric point at 7.0, 7.6 and 8.0, and an unresolved large spot at 29-30 kDa and with pI at 7.5-8.0, are obtained, may be derived from the aggregation of smaller beta-keratin proteins. The ultrastructural immunolocalization with the antibody against lizard beta-keratin shows that only small and large beta-keratin filaments of beta-cells of lizard epidermis are labeled. Keratin bundles in oberhautchen cells are less immunolabeled. Beta-keratin is rapidly polymerized into beta-packe...Continue Reading

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Sep 6, 2003·Journal of Experimental Zoology. Part B, Molecular and Developmental Evolution·Roger H SawyerLoren W Knapp
Jan 7, 2005·The Anatomical Record. Part A, Discoveries in Molecular, Cellular, and Evolutionary Biology·Lorenzo Alibardi, Mattia Toni
Apr 7, 2005·Cells, Tissues, Organs·Lorenzo Alibardi, Mattia Toni
Oct 12, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Luisa Dalla ValleLorenzo Alibardi

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Citations

Jul 14, 2010·Journal of Comparative Physiology. A, Neuroethology, Sensory, Neural, and Behavioral Physiology·Marie-Christin G KleinStanislav N Gorb
Aug 17, 2012·Journal of the Royal Society, Interface·Marie-Christin G Klein, Stanislav N Gorb
Mar 15, 2012·Science China. Life Sciences·Ce GuoZhendong Dai
Apr 9, 2008·Progress in Histochemistry and Cytochemistry·Lorenzo Alibardi, Mattia Toni
Dec 16, 2006·Zoology : Analysis of Complex Systems, ZACS·Mattia Toni, Lorenzo Alibardi
Apr 26, 2016·The Anatomical Record : Advances in Integrative Anatomy and Evolutionary Biology·Ahmed A AllamRasha E Abo-Eleneen

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