The major cell surface glycoprotein of chick embryo fibroblasts, cellular fibronectin (formerly known as CSP or LETS protein), was purified and used to produce monospecific antisera. After affinity purification, the anti-fibronectin was used to investigate fibronectin's localization, its transfer from intracellular to extracellular pools, its antibody-induced redistribution on the cell surface, and its role in cell shape. Anti-fibronectin localizes to extracellular fibrils located under and between sparse cells, and to a dense matrix that surrounds confluent cells. Cellular fibronectin is also present in granular intracytoplasmic structures containing newly synthesized fibronectin before secretion. This intracellular staining disappears 2 h after treatment with cycloheximide or puromycin, and returns after removal of these protein synthesis inhibitors. In pulse-chase experiments using cycloheximide, fibronectin was sequentially transferred from the intracellular to the fibrillar extracellular forms. Transformation of chick fibroblasts results in decreases in both extracellular and intracellular fibronectin, and in altered cell shape. Treatment of untransformed chick fibroblasts with anti-fibronectin results in rapid (30 min) al...Continue Reading
Alterations in surface proteins in chicken cells transformed by temperature-sensitive mutants of Rous sarcoma virus
Fibroblast surface antigen (SF): the external glycoprotein lost in proteolytic stimulation and maligant transfromation
Cell surface protein partially restores morphology, adhesiveness, and contact inhibition of movement to transformed fibroblasts
Mechanism of the decrease in the major cell surface protein of chick embryo fibroblasts after transformation
Changes in the distribution of a major fibroblast protein, fibronectin, during mitosis and interphase
Quantitation of a transformation-sensitive, adhesive cell surface glycoprotein. Decrease of several untransformed permanent cell lines
Mobility and distribution of a cell surface glycoprotein and its interaction with other membrane components
Transmembrane interactions and the mechanism of capping of surface receptors by their specific ligands
Microfilament bundles and cell shape are related to adhesiveness to substratum and are dissociable from growth control in cultured fibroblasts
Cytochalasin B releases a major surface-associated glycoprotein, fibronectin, from cultured fibroblasts
In vitro traits of adenovirus-transformed cell lines and their relevance to tumorigenicity in nude mice
Surface distribution of LETS protein in relation to the cytoskeleton of normal and transformed cells
Correlation between tumor induction and the large external transformation sensitive protein on the cell surface
The effects of reciprocal changes in temperature on the transformed state of cells infected with a rous sarcoma virus mutant
Changes in a surface-labelled galactoprotein and in glycolipid concentrations in cells transformed by a temperature-sensitive polyoma virus mutant
Distribution of fibroblast surface antigen: association with fibrillar structures of normal cells and loss upon viral transformation
Antigen cap formation in cultured fibroblasts: a reflection of membrane fluidity and of cell motility
Plasma membrane and internalized immunoglobulins of lymph node cells studied with conjugates of antibody or its Fab fragments with horseradish peroxidase
Bisdiamine inhibits extracellular matrix formation and cell proliferation of atrioventricular mesenchyme from developing chick heart valves
Stereomorphologic observation of bone tissue response to hydroxyapatite using SEM with the EDTA-KOH method
Distribution of surface fibronectin in low- and high-density cultures of normal and transformed mouse fibroblasts
Contamination of a commercial antifibronectin antiserum by antibodies directed against glycosaminoglycan determinants. A hypothesis
Cultured sympathetic neurons: effects of cell-derived and synthetic substrata on survival and development
Monoclonal antibody to human fibronectin: production and characterization using human muscle cultures
Immunofluorescent localization of collagens, fibronectin, and laminin during terminal differentiation of odontoblasts
Distribution of latex beads and retinal pigment epithelial cells along the ventral neural crest pathway
Expression of different regional patterns of fibronectin immunoreactivity during mesoblast formation in the chick blastoderm
Regulation of proliferation of embryonic heart mesenchyme: role of transforming growth factor-beta 1 and the interstitial matrix
Proteolytic activity of arginine esterase from dog seminal plasma towards actin and other structural proteins. Comparison with trypsin and kallikrein
Preliminary studies on the phenomenological behaviour of osteoblasts cultured on hydroxyapatite ceramics
The initial expression and patterned appearance of tenascin in scutate scales is absent from the dermis of the scaleless (sc/sc) chicken
Mr 205,000 sulfoglycoprotein in extracellular matrix of mouse fibroblast cells is immunologically related to high molecular weight microtubule-associated proteins
Fluorescent gangliosides as probes for the retention and organization of fibronectin by ganglioside-deficient mouse cells
The extracellular matrix of normal chick embryo fibroblasts: its effect on transformed chick fibroblasts and its proteolytic degradation by the transformants
Fibrillar organization of fibronectin is expressed coordinately with cell surface gangliosides in a variant murine fibroblast
Regulation of fibronectin receptor distribution by transformation, exogenous fibronectin, and synthetic peptides
The fibronectin receptor is organized by extracellular matrix fibronectin: implications for oncogenic transformation and for cell recognition of fibronectin matrices
Analysis of fibronectin receptor function with monoclonal antibodies: roles in cell adhesion, migration, matrix assembly, and cytoskeletal organization
An instructive role for the interstitial matrix in tissue patterning: tissue segregation and intercellular invasion
Cellular tumorigenicity in nude mice. Test of associations among loss of cell-surface fibronectin, anchorage independence, and tumor-forming ability
alpha 2 Macroglobulin binding to the plasma membrane of cultured fibroblasts. Diffuse binding followed by clustering in coated regions
Distribution of fetal bovine serum fibronectin and endogenous rat cell fibronectin in extracellular matrix
Absence of fibronectin and presence of plasminogen activator in both normal and malignant human mammary epithelial cells in culture
Immunocytochemical localization of procollagen and fibronectin in human fibroblasts: effects of the monovalent ionophore, monensin
Human alveolar macrophage fibronectin: synthesis, secretion, and ultrastructural localization during gelatin-coated latex particle binding
Role of fibronectin in collagen deposition: Fab' to the gelatin-binding domain of fibronectin inhibits both fibronectin and collagen organization in fibroblast extracellular matrix
Cell surface molecules and fibronectin-mediated cell adhesion: effect of proteolytic digestion of membrane proteins
A cell surface integral membrane glycoprotein of 85,000 mol wt (gp85) associated with triton X-100-insoluble cell skeleton
Identification of fibronectins in peritoneal macrophages during the phagocytosis of Brucella. An immunocytochemical study by electron microscopy
Fibronectin in the tendon-synovial complex: quantitation in vivo and in vitro by ELISA and relative mRNA levels by polymerase chain reaction and northern blot
Transformation-sensitive cell surface protein: isolation, characterization, and role in cellular morphology and adhesion
Kupffer cells from CC1(4)-treated rat livers induce skin fibroblast and liver fat-storing cell proliferation in culture
An anticatalytic monoclonal antibody to avian plasminogen activator: its effect on behavior of RSV-transformed chick fibroblasts
Cell adhesion and metastasis: is the site specificity of cancer metastasis determined by leukocyte-endothelial cell recognition and adhesion?
Proteolytic activity of specialized surface protrusions formed at rosette contact sites of transformed cells
Evidence for an interaction between the cell surface and intermediate filaments in cultured fibroblasts
The immunoreactivity, ligand, and cell binding characteristics of rheumatoid synovial fluid fibronectin
Comparisons of evolutionarily distinct fibronectins: evidence for the origin of plasma and fibroblast cellular fibronectins from a single gene
Changes in the extracellular matrix components fibronectin and laminin during immune rejection of skeletal muscle
Sodium butyrate affects expression of fibronectin on CHO cells: specific increase in antibody-complement-mediated cytotoxicity
Variant endothelial cells. Fibronectin as a transducer of signals for migration and neovascularisation
Spreading of human fibroblasts in serum-free medium: inhibition by dithiothreitol and the effect of cold insoluble globulin (plasma fibronectin)
Occurrence of fibronectin on the primary mesenchyme cell surface during migration in the sea urchin embryo
Structure of integrin, a glycoprotein involved in the transmembrane linkage between fibronectin and actin
Immunochemical and ultrastructural characterization of serum proteins associated with monosodium urate crystals (MSU) in synovial fluid cells from patients with gout
Analysis of the suitability of calreticulin inducible HEK cells for adhesion studies: microscopical and biochemical comparisons
Preliminary studies of PVA/PVP blends incorporated with HAp and β-TCP bone ceramic as template for hard tissue engineering
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