PMID: 6998383Jan 1, 1980

Immunological characterization of fibrous protein isolated from human stratum corneum

Archives of Dermatological Research
H Ogawa, M Hattori

Abstract

Specific antibody was produced against the purified fibrous protein isolated from human stratum corneum. Two precipitin lines appeared when the antiserum was tested against fibrous protein of human stratum corneum (FP/SC). When the section of human plantar skin was incubated with antihuman FP/SC serum for 30 min at 37 degrees C for indirect immunofluorescent studies, only a poor fluorescence could be detected in the living layers. The fluorescence in these layers increased on the section that was incubated with the fluorescence in these layers increased on the section that was incubated with the antiserum for 1 h at 37 degrees C. However, no fluorescence could be observed in the stratum corneum under these conditions. On the other hand, irregular fluorescence appeared in the stratum corneum after more prolonged incubation (2 h) with antiserum at 37 degrees C. The fluorescence also appeared in the stratum corneum when the section was incubated with antiserum for 30 min following a short pretreatment with urea-buffer. Identical precipitin lines were observed on double diffusion of anti-human FP/SC with prekeratin and 0.85% NaCl-Tris acetate buffer (pH 7.5) soluble proteins of human living layers.

References

May 1, 1979·The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society·H OgawaT Sekine
Aug 1, 1978·The Journal of Investigative Dermatology·H P Baden, L D Lee
Nov 18, 1975·Biochimica Et Biophysica Acta·L D LeeH P Baden
Oct 1, 1976·The Journal of Investigative Dermatology·L D LeeB F Fleming
Nov 1, 1976·The Journal of Investigative Dermatology·H P BadenJ Kubilus
Jul 1, 1975·The Biochemical Journal·P M Steinert

Related Concepts

SDS-PAGE
Immunofluorescence Assay
Foot
Prausnitz-Kustner Test
Gene Products, Protein
Skin

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Pediculosis pubis

Pediculosis pubis is a disease caused by a parasitic insect known as Pthirus pubis, which infests human pubic hair, as well as other areas with hair including eye lashes. Here is the latest research.

Rh Isoimmunization

Rh isoimmunization is a potentially preventable condition that occasionally is associated with significant perinatal morbidity or mortality. Discover the latest research on Rh Isoimmunization here.

Genetic Screens in iPSC-derived Brain Cells

Genetic screening is a critical tool that can be employed to define and understand gene function and interaction. This feed focuses on genetic screens conducted using induced pluripotent stem cell (iPSC)-derived brain cells. It also follows CRISPR-Cas9 approaches to generating genetic mutants as a means of understanding the effect of genetics on phenotype.

Enzyme Evolution

This feed focuses on molecular models of enzyme evolution and new approaches (such as adaptive laboratory evolution) to metabolic engineering of microorganisms. Here is the latest research.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Pharmacology of Proteinopathies

This feed focuses on the pharmacology of proteinopathies - diseases in which proteins abnormally aggregate (i.e. Alzheimer’s, Parkinson’s, etc.). Discover the latest research in this field with this feed.

Alignment-free Sequence Analysis Tools

Alignment-free sequence analyses have been applied to problems ranging from whole-genome phylogeny to the classification of protein families, identification of horizontally transferred genes, and detection of recombined sequences. Here is the latest research.