PMID: 6978173Apr 15, 1982Paper

Immunological characterization of non-Hodgkin's lymphoma: enrichment of neoplastic cells from lymphoid tissues and blood

Cancer
A A Bom-Van NoorloosC J Melief

Abstract

Cell suspensions prepared from lymph nodes, spleen or peripheral blood of patients with non-Hodgkin's lymphoma (NHL) often contain a high percentage of residual nonmalignant cellular elements. By E-rosette sedimentation, it was possible to enrich such suspensions from patients with various types of lymphoma for malignant cells. In patients with a B- or non-B/non-T-cell lymphoma, the neoplastic cells were found in the non-T fraction. The capacity to respond to stimulation by various stimuli was then confined to the T-cell fraction, which contained the residual normal T-cells. In patients with T-cell lymphomas, in which the malignant cells had retained the capacity to form E-rosettes, lymphoma cells were found in the T fraction. These cells usually did not respond to mitogenic stimuli. Using this separation method, small proportions of neoplastic cells could be identified in mixed cell populations. Thus, in the blood from nine out of 23 lymphoma patients without abnormalities in routine blood tests, a population of abnormal cells was detected after cell separation. This included a monoclonal B-cell population in the blood of four patients, a questionably monoclonal B-cell population in the blood of two patients and in increased n...Continue Reading

References

Jun 15, 1977·Cellular Immunology·M H van Oers, W P Zeijlemaker
Jun 1, 1976·Scandinavian Journal of Immunology·M J Du BoisV P Eijsvoogel
Jan 1, 1979·International Archives of Allergy and Applied Immunology·M H van OersW P Zeijlemaker
Jul 1, 1979·British Journal of Cancer·J A HabeshawR L Brearley
Mar 1, 1977·European Journal of Immunology·M H van OersP T Schellekens
May 1, 1979·British Journal of Haematology·J V GarrettR K Newton
Apr 17, 1975·The New England Journal of Medicine·L Sen, L Borella
Jan 1, 1973·Tissue Antigens·M G Du BoisV P Eijsvoogel
Aug 1, 1972·The Journal of Experimental Medicine·S S Fröland, J B Natvig
Apr 24, 1980·The New England Journal of Medicine·A A Bom-van NoorloosC J Melief
Apr 1, 1980·Cell·E L Reinherz, S F Schlossman

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