PMID: 6786331Apr 28, 1981Paper

Immunological comparison of rat, rabbit, and human microsomal cytochromes P-450

Biochemistry
F P GuengerichM B Mitchell

Abstract

Antibodies were raised in rabbits to electrophoretically homogeneous cytochromes P-450 isolated from rat and human liver microsomes. These antibodies were used to compare various forms of rat, rabbit, and human cytochromes P-450 present in microsomes and in purified preparations by using double-diffusion analysis, immunoelectrophoresis, quantitative microcomplement fixation, competitive radioimmune assay and inhibition of enzyme activity toward d-benzphetamine and benzo[a]pyrene. The results indicate that (1) at least some forms of cytochrome P-450 from the three species share certain common immunological determinants, (2) there are immunological differences between cytochromes P-450 isolated from the three species, (3) some immunological differences exist between cytochromes P-450 isolated from rats of different strains, (4) immunologically distinguishable forms of cytochrome P-450 exist within individual human liver samples, and (5) human liver samples obtained from different individuals contain immunologically different forms of cytochrome P-450. Quantitative microcomplement fixation techniques were used to assign immunological distances to different form of rat, rabbit, and human liver microsomal cytochrome P-450. Cross-rea...Continue Reading

References

Jan 1, 1979·Drug Metabolism Reviews·A Y Lu
Sep 1, 1979·Proceedings of the National Academy of Sciences of the United States of America·H TowbinJ Gordon
Jan 1, 1979·Pharmacology & Therapeutics·F P Guengerich
Dec 25, 1978·Life Sciences·J BaronF P Guengerich
Oct 1, 1976·Analytical Biochemistry·T MatsubaraK Sugeno
Jan 1, 1980·Archives of Biochemistry and Biophysics·P WangF P Guengerich
Apr 1, 1965·Proceedings of the Society for Experimental Biology and Medicine·R L CRAMJ R FOUTS

Citations

Jun 5, 1993·Biotechnology and Bioengineering·P Y Huang, C S Lee
May 1, 1987·Proceedings of the National Academy of Sciences of the United States of America·M K ReddyJ K Reddy
Jan 1, 1988·Journal of Toxicology and Environmental Health·C D Carrington, M B Abou-Donia
Sep 1, 1983·Archives of Biochemistry and Biophysics·C B PickettA Y Lu
May 1, 1982·Archives of Biochemistry and Biophysics·F P Guengerich, N K Davidson
Jun 1, 1984·Archives of Biochemistry and Biophysics·P I MackenzieI S Owens
May 1, 1986·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·F P GuengerichM V Martin
Nov 1, 1982·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·F P GuengerichL S Kaminsky
Jan 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·E F Johnson, G E Schwab
Jan 1, 1984·Xenobiotica; the Fate of Foreign Compounds in Biological Systems·A R Boobis, D S Davies
Jan 1, 1982·Quarterly Reviews on Drug Metabolism and Drug Interactions·R MakowskiG G Gibson

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