PMID: 6163471Jan 30, 1981Paper

Immunological cross-reactivity between chicken slow skeletal and ventricular muscle myosin

Biochimica Et Biophysica Acta
S Sartore

Abstract

Antibodies elicited in rabbits against chicken slow skeletal anterior latissimus dorsi and ventricular myosin were analyzed by double immunodiffusion for their ability to react with homologous and heterologous antigen at different stages of immunization (1--12 months). Each anti myosin antiserum formed a single, strong precipitin line with its immunogen after short time of immunization. This reaction was specific for myosin heavy chains as determined by GEDELISA (gel electrophoresis derived enzyme lined immunosorbent assay) test. In rabbits injected with ventricular myosin after long time of immunization a second, fainter precipitin line has generally been observed. The antigenic determinants responsible for this precipitin line have been localized on the light myosin subunits. By comparing the two types of anti myosin antisera with heterologous antigen we have obtained evidence for partial immunological cross-reactivity between slow skeletal and ventricular muscle myosins. In particular, all anti ventricular myosin antisera displayed a marked immunological reactivity with anterior latissimus dorsi myosin whereas most of anti anterior latissimus dorsi myosin antisera showed absence of reciprocity. By means of immunofluorescence...Continue Reading

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Citations

May 1, 1984·Proceedings of the National Academy of Sciences of the United States of America·D J FriedmanM Rabinowitz
Feb 1, 1983·Experimental Cell Research·L GorzaS Schiaffino
Apr 1, 1987·Journal of Muscle Research and Cell Motility·S SartoreS Schiaffino
Jun 29, 1981·Biochimica Et Biophysica Acta·L Dalla Libera, S Sartore

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