Immunological cross-reactivity between rainbow trout GTH I and GTH II and their alpha and beta subunits: application to the development of specific radioimmunoassays

General and Comparative Endocrinology
M GovorounB Breton

Abstract

Immunological cross-reactivities between rainbow trout GTH I and GTH II and their alpha and beta have been studied using highly purified rainbow trout gonadotropins and subunits and antibodies raised against beta subunits. From these observations radioimmunoassays have been developed for rainbow trout GTH I and GTH II. The GTH II RIA was highly specific and cross-reacted only with GTH II and its beta 1 subunits, with beta 2 being less potent than beta 1 in competing GTH II binding. There was no cross-reactivity with GTH I. Its sensitivity varied between 0.1 and 0.2 ng/ml, allowing GTH II measurement early in the reproductive cycle. Variations between and within assays were less than 10%. There was a lack of specificity of GTH I RIA (44% cross-reactivity with GTH II, when using labelled native GTH I). Reasons for this lack of sensitivity were studied. It cannot be attributed to beta subunits (less than 1.2% cross-reactivity). However, the cross-reactivity of alpha subunits was very important. This suggests that the presence of free alpha subunits in the medium can be responsible for the lack of specificity. Labelling native GTH I resulted in conformational change in molecular weight and dissociation of the hormone into subunits,...Continue Reading

References

Sep 1, 1988·General and Comparative Endocrinology·K SuzukiH Kawauchi
Dec 9, 1998·Journal of Molecular Endocrinology·J T Dickey, P Swanson
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Citations

Aug 19, 2009·General and Comparative Endocrinology·B Levavi-SivanJ J Lareyre
May 16, 2009·General and Comparative Endocrinology·Geir Lasse TarangerTom Hansen
Nov 1, 2015·General and Comparative Endocrinology·Mitsuo NyujiKoichi Okuzawa
Jul 27, 2007·Journal of Experimental Zoology. Part A, Ecological Genetics and Physiology·Masafumi AmanoShoji Kitamura
May 15, 2001·The Journal of Experimental Zoology·M AmanoK Aida
Jul 21, 2020·General and Comparative Endocrinology·G MolésA Gómez

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