PMID: 1064879Jun 1, 1976Paper

Immunological cross-reactivity of antibodies to a synthetic undecapeptide analogous to the amino terminal segment of carcinoembryonic antigen, with the intact protein and with human sera

Proceedings of the National Academy of Sciences of the United States of America
R ArnonM Sela

Abstract

A peptide corresponding to the 11 amino acid residues of the NH2-terminal portion in the sequence of carcinoembryonic antigen(CNTHETIC CEA(1-11) peptide was attached by means of a water-soluble carbodiimide reagent to multichain poly(DL-alanine( as well as to bovine serum albumin. Both macromolecular conjugates provoked in rabbit anti-CEA(1-11) peptide antibodies. The specificity of this immunological system and the crossreactivity between the peptide and intact CEA were investigated by two methods--passive hemagglutination and modified bacteriophage inactivation. Hemmagglutination experiments showed that not only anti-CEA(1-11) sera, but also anti-CEA sera, agglutinated CEA(1-11)-coated sheep erythrocytes, and both these reactions were inhibited with CEA(1-11) peptide. In experiments with the chemically modified bacteriophage technique CEA(1-11)-coated phase was efficiently inactivated with antisera against the CEA(1-11) conjugates, and the inactivation reaction could be totally inhibited with the free peptide. The semipure CEA, but not the pure protein, could also inhibit the phage inactivation, even though less efficiently. On the basis of the above results, sera of some cancer patients were tested for their capacity to inhi...Continue Reading

References

Sep 1, 1972·Proceedings of the National Academy of Sciences of the United States of America·S von KleistP Burtin
Sep 1, 1973·Immunochemistry·J E ColiganW D Terry
Sep 1, 1968·The Journal of Experimental Medicine·J KrupeyS O Freedman
Mar 1, 1973·Immunochemistry·G Pusztaszeri, J P Mach
Sep 13, 1972·Nature: New Biology·J M GoldP Gold
Apr 1, 1972·Immunochemistry·J E ColiganC W Todd
Feb 1, 1973·Cancer·R DelwicheN Marcon
Sep 1, 1965·The Journal of Experimental Medicine·P Gold, S O Freedman
Jul 1, 1972·The Journal of Experimental Medicine·W D TerryC W Todd
Mar 1, 1972·Immunochemistry·M L EganC W Todd
Jan 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·R Arnon, M SELA
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·D M ThomsonP Gold
Jul 1, 1971·Proceedings of the National Academy of Sciences of the United States of America·R ArnonC B Anfinsen
Apr 28, 1970·Biochimica Et Biophysica Acta·J HaimovichM SELA

Citations

Sep 10, 1962·Biochimica Et Biophysica Acta·D GIVOLM SELA
Nov 1, 1980·Proceedings of the National Academy of Sciences of the United States of America·R ArnonL Chedid
Jul 29, 1983·Journal of Immunological Methods·R ArnonC O Jacob
Jan 1, 1985·Critical Reviews in Oncology/hematology·J E Shively, J D Beatty
Sep 19, 2003·The Journal of Biological Chemistry·M SELA

Related Concepts

Tumor Antibodies
Carcinoembryonic Antigen
Cross Reactions
Malignant Neoplasms
Oligopeptides

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