Immunological detection of in vivo aflatoxin B1-DNA adduct formation in rats, rainbow trout and coho salmon

Carcinogenesis
Y NakatsuruT Ishikawa

Abstract

Immunological detection of carcinogen-DNA adducts in organs or tissues should prove a particularly useful approach for monitoring carcinogen exposure, for characterization of carcinogen binding to DNA and for investigating DNA repair processes in vivo. In one of a series of experiments aimed at raising antibodies against several carcinogen-modified DNAs, rabbits were immunized in our laboratory with aflatoxin B1 (AFB1)-modified DNA. After the titer and specificity of the antibodies produced were checked against standards by the enzyme-linked immunosorbent assay (ELISA) method, they were used to investigate DNA extracted from livers of rats and rainbow trout (Salmo mykiss) injected with tritium-labeled AFB1 at doses of 1-2 mg/kg (rats) or 0.1-0.5 mg/kg (rainbow trout). Adduct levels were compared using both radioactivity and ELISA methods. Positive DNA binding could be detected in both rats and rainbow trout by the immunological method at similar levels to those estimated with the radioactive analysis. To throw light on possible mechanisms underlying the wide variation in response to aflatoxins among salmonid fish, DNA extracted from the livers of rainbow trout (susceptible species) and coho salmon (Oncorhynchus kisutch) (less s...Continue Reading

Citations

Apr 11, 2013·Aquatic Toxicology·Aude KienzlerAlain Devaux
Dec 15, 2016·Food Additives & Contaminants. Part A, Chemistry, Analysis, Control, Exposure & Risk Assessment·E C MichelinA M Fernandes
Jan 1, 1997·Biomarkers : Biochemical Indicators of Exposure, Response, and Susceptibility to Chemicals·W Pfau
Aug 9, 2018·PloS One·Carolina M Bedoya-SernaAndrezza M Fernandes

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