Immunological detection of isoferritins in normal human serum and tissue

Clinica Chimica Acta; International Journal of Clinical Chemistry
L V McKeeringL W Powell


A method is described which permits the detection of isoferritins in normal human serum and tissues. The technique makes use of 125I-labelled monospecific anti-human-liver-ferritin antibody to demonstrate the isoferritins after isoelectric focussing of the purified ferritin in polyacrylamide gels. The organ-specific variation in tissue isoferritin profile previously reported in normal subjects has been confirmed by this technique using only 50 ng of each ferritin sample. Serum ferritin from normal healthy subjects was also shown to exhibit a microheterogeneity on isoelectric focussing; six clearly defined isoferritin peaks were detected in the pH range of 5.04 to 5.62. This isoferritin profile of normal serum contained isoferritins over the whole range of the various tissue isoferritins suggesting that a number of organs may contribute to the normal serum ferritin pool.


May 1, 1975·British Journal of Haematology·L W PowellJ W Drysdale
May 1, 1975·The New England Journal of Medicine·A Jacobs, M Worwood
Mar 16, 1973·Nature·E AlpertJ W Drysdale
Sep 1, 1974·British Journal of Haematology·M A Siimes, P R Dallman
Feb 1, 1973·Analytical Biochemistry·G R Schacterle, R L Pollack


Aug 1, 1979·British Journal of Haematology·J W HallidayL W Powell
Oct 1, 1984·Kidney International·F L Van de VyverM E De Broe
Jul 31, 2013·Biochimica Et Biophysica Acta·Ahmed A Alkhateeb, J R Connor
Sep 15, 1977·International Journal of Cancer. Journal International Du Cancer·E J SarcioneL Stutzman
Jan 1, 1988·Annals of the New York Academy of Sciences·J W Halliday, L W Powell
Sep 1, 1983·British Journal of Haematology·G D Blight, E H Morgan
Oct 1, 1983·British Journal of Haematology·H P RoeserB E Cham
Jul 1, 1980·British Journal of Haematology·H P RoeserD Willgoss
Apr 27, 2007·American Journal of Physiology. Cell Physiology·J FisherJ R Connor
Jan 1, 1978·CRC Critical Reviews in Clinical Laboratory Sciences·C P Alfrey
Jan 1, 1983·Critical Reviews in Clinical Laboratory Sciences·G D McLarenR W Kellermeyer
May 3, 2002·Blood·Frank M Torti, Suzy V Torti
Apr 25, 2020·Microorganisms·Ana C MoreiraMaria Salomé Gomes

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