Immunological detection of isoferritins in normal human serum and tissue

Clinica Chimica Acta; International Journal of Clinical Chemistry
L V McKeeringL W Powell

Abstract

A method is described which permits the detection of isoferritins in normal human serum and tissues. The technique makes use of 125I-labelled monospecific anti-human-liver-ferritin antibody to demonstrate the isoferritins after isoelectric focussing of the purified ferritin in polyacrylamide gels. The organ-specific variation in tissue isoferritin profile previously reported in normal subjects has been confirmed by this technique using only 50 ng of each ferritin sample. Serum ferritin from normal healthy subjects was also shown to exhibit a microheterogeneity on isoelectric focussing; six clearly defined isoferritin peaks were detected in the pH range of 5.04 to 5.62. This isoferritin profile of normal serum contained isoferritins over the whole range of the various tissue isoferritins suggesting that a number of organs may contribute to the normal serum ferritin pool.

References

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Citations

Aug 1, 1979·British Journal of Haematology·J W HallidayL W Powell
Oct 1, 1984·Kidney International·F L Van de VyverM E De Broe
Jul 31, 2013·Biochimica Et Biophysica Acta·Ahmed A Alkhateeb, J R Connor
Sep 15, 1977·International Journal of Cancer. Journal International Du Cancer·E J SarcioneL Stutzman
Jan 1, 1988·Annals of the New York Academy of Sciences·J W Halliday, L W Powell
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May 3, 2002·Blood·Frank M Torti, Suzy V Torti
Apr 25, 2020·Microorganisms·Ana C MoreiraMaria Salomé Gomes

Related Concepts

Isoferritin
Hydrogen-Ion Concentration
Iodine Radioisotopes
Isoelectric Focusing
Kidney
Liver
Myocardium
Tissue Specificity
Pancreas
Radioimmunoassay

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