Abstract
Neisseria meningitidis group C polysaccharide-tetanus toxoid conjugate was prepared to obtain the polysaccharide component in a thymus-dependent form and to preserve the immunogenic properties of the tetanus toxoid component. Biochemical and immunochemical analyses of this conjugate revealed that (i) it was composed of equal amounts of polysaccharide and protein; (ii) the antigenic activity of the polysaccharide component was greatly reduced; (iii) it contained about 10% free polysaccharide; (iv) the composition was not homogeneous; and (v) only 5% of the tetanus toxoid component was present at the surface of the conjugate molecules. In this study, the influence of these characteristics on the antibody response to both components in mice was investigated. The dose-response relationship, the persistence of antibodies, a possible antigenic competition, and the specificities of the antibodies induced were also studied. Our data suggest that the conjugate behaves as a pronounced thymus-dependent antigen, that the tetanus toxoid component is more immunogenic at lower dosages (0.8 and 20 ng) than equivalent doses of tetanus vaccine, that the presence of free polysaccharide does not influence the induction of antibodies to polysacchar...Continue Reading
References
Aug 1, 1975·The Journal of Experimental Medicine·J CisarJ Liao
Jun 1, 1979·Infection and Immunity·J G VosA M Hagenaars
Mar 1, 1970·The Journal of Experimental Medicine·R B Roberts
Jul 1, 1972·Physiological Reviews·M Potter
Jan 1, 1973·Current Topics in Microbiology and Immunology·M J Taussig
Jan 1, 1974·Advances in Immunology·H F Pross, D Eidinger
Jun 1, 1969·The Journal of Experimental Medicine·I GoldschneiderM S Artenstein
Jun 1, 1969·The Journal of Experimental Medicine·I GoldschneiderM S Artenstein
Feb 1, 1968·The Journal of Experimental Medicine·I Schechter
Oct 1, 1981·Cellular Immunology·D Wilson, H Braley-Mullen
Feb 1, 1983·The Journal of Experimental Medicine·K E SteinW E Paul
Apr 1, 1983·Infection and Immunity·E C BeuveryJ Haverkamp
Jan 1, 1983·Infection and Immunity·P Anderson
Apr 1, 1983·Infection and Immunity·C ChuS C Rastogi
Dec 1, 1981·Infection and Immunity·J M GriffissS L Berman
Jul 1, 1982·Infection and Immunity·E C BeuveryJ Nagel
Aug 1, 1982·Infection and Immunity·E C BeuveryJ Nagel
Apr 1, 1980·The Journal of Experimental Medicine·J SlackJ M Davie
Aug 1, 1980·The Journal of Experimental Medicine·R SchneersonJ B Robbins
Sep 1, 1963·Proceedings of the National Academy of Sciences of the United States of America·M J OSBORN
Citations
Mar 11, 2005·Expert Opinion on Biological Therapy·Carl E Frasch
Apr 27, 2000·The Pediatric Infectious Disease Journal·A J Pollard, M Levin
May 25, 2004·Infection and Immunity·Pablo A García-OjedaIan M Feavers
Dec 22, 1999·Infection and Immunity·P A García-OjedaK E Stein
Jul 24, 2008·Glycoconjugate Journal·Cyrille GrandjeanLaurence A Mulard
Nov 11, 2015·Clinical Infectious Diseases : an Official Publication of the Infectious Diseases Society of America·Carl E FraschJean-Marie Préaud
Jan 19, 2006·Clinical Microbiology Reviews·Lee H Harrison
May 3, 2011·Hybridoma·Hyeong-Jwa ChoiYoung-Sun Kang
Oct 24, 1998·Infection and Immunity·L J RubinsteinK E Stein
Feb 13, 2001·Vaccine·A J Pollard, C Frasch
Dec 24, 1996·Carbohydrate Research·X Lemercinier, C Jones
Mar 1, 1987·Vaccine·P A van de WielE C Beuvery
Nov 1, 1992·Infection and Immunity·D C WatsonS C Szu
Feb 1, 1985·Infection and Immunity·J W ZigtermanJ F Vliegenthart
Aug 28, 2007·Vaccine·I A F B SilveiraE Jessouroun
Dec 28, 2007·Vaccine·Africa González-FernándezCarmen Fernández