PMID: 6987661Feb 1, 1980Paper

Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus

Proceedings of the National Academy of Sciences of the United States of America
G Krohne, W W Franke

Abstract

Nuclei of vitellogenic oocytes of the frog, Xenopus laevis, contain a prominent protein, representing about 10% of nuclear protein, which is characterized by a polypeptide of M(r) 30,000. This protein is highly phosphorylated and acidic, displays several isoelectric variants with pI values ranging from 4.7 to 5.3, shows a high thermostability, is not stably complexed with other proteins, and is readily extracted in buffer solutions. Guinea pig antibodies against this protein have allowed its specific immunoprecipitation and localization by immunofluorescence microscopy, using both frozen tissue sections and cells grown in vitro. The protein is located almost exclusively in the nucleus where it appears to be spread throughout the nuclear interior. It is also a major nucleus-specific protein in vitellogenic and previtellogenic oocytes of other amphibian species as well as in other cell types, including hepatocytes, follicle epithelial cells, and cultured Xenopus cells, but is not detected in nuclei of transcriptionally inactive cells such as erythrocytes and spermatids. An immunologically related, if not identical, protein occurs in nuclei of higher vertebrate cells, including mammals. The properties of this abundant nuclear phos...Continue Reading

References

Jul 1, 1975·Proceedings of the National Academy of Sciences of the United States of America·J O Thomas, R D Kornberg
Mar 1, 1977·Proceedings of the National Academy of Sciences of the United States of America·I L Goldknopf, H Busch
Oct 1, 1978·Proceedings of the National Academy of Sciences of the United States of America·W W FrankeK Weber
Jun 1, 1979·The Journal of Cell Biology·W W FrankeK Weber
Dec 1, 1977·Cell·T G Clark, R W Merriam
Mar 16, 1978·Nature·E M De RobertisJ B Gurdon
Oct 1, 1978·Experimental Cell Research·G KrohneU Scheer
Mar 13, 1975·Nature·P L PaineS B Horowitz
Dec 1, 1970·Proceedings of the Royal Society of London. Series B, Containing Papers of a Biological Character·J B Gurdon
Jul 1, 1974·European Journal of Biochemistry·W M Bonner, R A Laskey

Citations

Jan 1, 1983·Molecular and Cellular Biochemistry·Anthony T Annunziato, R L Seale
Dec 1, 1989·Developmental Biology·M Luke, D F Bogenhagen
Dec 1, 1981·Mechanisms of Ageing and Development·Z A Medvedev
Dec 14, 1989·Biochimica Et Biophysica Acta·A CummingsJ Sommerville
Oct 12, 1999·International Journal of Biological Macromolecules·K IwataN Nishi
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C Dabauvalle, W W Franke
Dec 1, 1986·The Journal of Cell Biology·D NewmeyerD J Forbes
Feb 1, 1987·The Journal of Cell Biology·D R FinlayD J Forbes
Sep 1, 1988·The Journal of Cell Biology·R Duden, W W Franke
Mar 1, 1981·The Journal of Cell Biology·L Goldstein, C Ko
Jul 1, 1984·The Journal of Cell Biology·P L Paine
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·R F ZirwesW W Franke
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·C MertensW W Franke
Jun 1, 1997·International Journal of Biological Macromolecules·K IwataN Nishi
Jan 25, 2014·Biochimica Et Biophysica Acta·Anthony T Annunziato
May 1, 1984·Experimental Cell Research·M KellermayerC F Hazlewood
Sep 1, 1980·Experimental Cell Research·G Krohne, W W Franke
Oct 20, 2005·Progress in Neuro-psychopharmacology & Biological Psychiatry·Francine GouletNazrul Islam
Jan 1, 1986·CRC Critical Reviews in Biochemistry·R S WuW M Bonner
Jul 11, 2014·Biology of Reproduction·Aurélien BouleauJulien Bobe
Jan 1, 1985·Biochemistry·F D MillerJ H van de Sande
May 20, 1986·Biochemistry·L SealyR Chalkley
May 4, 1993·Biochemistry·A R SburlatiS L Berger
Apr 27, 2005·Archives of Biochemistry and Biophysics·Carme ArnanNúria Saperas
Aug 21, 1980·Nature·R A Laskey, W C Earnshaw
Mar 1, 1984·The Journal of Experimental Zoology·I M CrosbyR M Moor
Jan 1, 1982·CRC Critical Reviews in Biochemistry·I L CartwrightG C Howard
Oct 4, 1996·The Journal of Biological Chemistry·T ItoJ T Kadonaga
Oct 23, 1997·The Journal of Biological Chemistry·M W TrumboreS L Berger
Jan 1, 1989·Critical Reviews in Biochemistry and Molecular Biology·P Silver, H Goodson
Jul 1, 1981·Wilhelm Roux's Archives of Developmental Biology·Christine DreyerElisabeth Siegel
Nov 17, 1980·FEBS Letters·H R Woodland
Mar 29, 1996·The Journal of Biological Chemistry·G H LenoR A Laskey

Related Concepts

Amphibians
Cell Nucleus
Liver
Oocytes
Ovary

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Lipidomics & Rhinovirus Infection

Lipidomics can be used to examine the lipid species involved with pathogenic conditions, such as viral associated inflammation. Discovered the latest research on Lipidomics & Rhinovirus Infection.

Alzheimer's Disease: MS4A

Variants within the membrane-spanning 4-domains subfamily A (MS4A) gene cluster have recently been implicated in Alzheimer's disease in genome-wide association studies. Here is the latest research on Alzheimer's disease and MS4A.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Torsion Dystonia

Torsion dystonia is a movement disorder characterized by loss of control of voluntary movements appearing as sustained muscle contractions and/or abnormal postures. Here is the latest research.

Generating Insulin-Secreting Cells

Reprogramming cells or using induced pluripotent stem cells to generate insulin-secreting cells has significant therapeutic implications for diabetics. Here is the latest research on generation of insulin-secreting cells.

Central Pontine Myelinolysis

Central Pontine Myelinolysis is a neurologic disorder caused most frequently by rapid correction of hyponatremia and is characterized by demyelination that affects the central portion of the base of the pons. Here is the latest research on this disease.

Epigenome Editing

Epigenome editing is the directed modification of epigenetic marks on chromatin at specified loci. This tool has many applications in research as well as in the clinic. Find the latest research on epigenome editing here.