PMID: 6987661Feb 1, 1980Paper

Immunological identification and localization of the predominant nuclear protein of the amphibian oocyte nucleus

Proceedings of the National Academy of Sciences of the United States of America
G Krohne, W W Franke

Abstract

Nuclei of vitellogenic oocytes of the frog, Xenopus laevis, contain a prominent protein, representing about 10% of nuclear protein, which is characterized by a polypeptide of M(r) 30,000. This protein is highly phosphorylated and acidic, displays several isoelectric variants with pI values ranging from 4.7 to 5.3, shows a high thermostability, is not stably complexed with other proteins, and is readily extracted in buffer solutions. Guinea pig antibodies against this protein have allowed its specific immunoprecipitation and localization by immunofluorescence microscopy, using both frozen tissue sections and cells grown in vitro. The protein is located almost exclusively in the nucleus where it appears to be spread throughout the nuclear interior. It is also a major nucleus-specific protein in vitellogenic and previtellogenic oocytes of other amphibian species as well as in other cell types, including hepatocytes, follicle epithelial cells, and cultured Xenopus cells, but is not detected in nuclei of transcriptionally inactive cells such as erythrocytes and spermatids. An immunologically related, if not identical, protein occurs in nuclei of higher vertebrate cells, including mammals. The properties of this abundant nuclear phos...Continue Reading

References

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Citations

Jan 1, 1983·Molecular and Cellular Biochemistry·A T Annunziato, R L Seale
Dec 1, 1989·Developmental Biology·M Luke, D F Bogenhagen
Dec 1, 1981·Mechanisms of Ageing and Development·Z A Medvedev
Dec 14, 1989·Biochimica Et Biophysica Acta·A CummingsJ Sommerville
Oct 12, 1999·International Journal of Biological Macromolecules·K IwataN Nishi
Sep 1, 1982·Proceedings of the National Academy of Sciences of the United States of America·M C Dabauvalle, W W Franke
Dec 1, 1986·The Journal of Cell Biology·D D NewmeyerD J Forbes
Feb 1, 1987·The Journal of Cell Biology·D R FinlayD J Forbes
Sep 1, 1988·The Journal of Cell Biology·R Duden, W W Franke
Mar 1, 1981·The Journal of Cell Biology·L Goldstein, C Ko
Jul 1, 1984·The Journal of Cell Biology·P L Paine
Oct 23, 1997·Proceedings of the National Academy of Sciences of the United States of America·R F ZirwesW W Franke
Jun 21, 2001·Proceedings of the National Academy of Sciences of the United States of America·C MertensW W Franke
Jun 1, 1997·International Journal of Biological Macromolecules·K IwataN Nishi
Jan 25, 2014·Biochimica Et Biophysica Acta·Anthony T Annunziato
May 1, 1984·Experimental Cell Research·M KellermayerC F Hazlewood
Sep 1, 1980·Experimental Cell Research·G Krohne, W W Franke

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