Immunological properties of glycolipids from membranes of Acholeplasma laidlawii.

Infection and Immunity
M D RyanL L Matz

Abstract

Glycolipids, the predominant class of lipids in the membranes of Acholeplasma laidlawii, are the haptenic determinants that react with anti-A. Laidlawii serum to fix complement. The predominant complement-fixing activity of the membrane glycolipids was associated with the monoglucoysyl diglyceride, diglucosyl diglyceride, glycerlphosphoryl diglucosyl diglyceride (GPDD), and an unknown lipid B, which did not react with ninhydrin but release glucose and glycerol and traces of phosphorus upon hydrolysis. The glycolipids monoglucosyl diglyceride and diglucosyl diglyceride or GPDD and unknown lipid B were paired as a result of their cross-reactions with selective antisera prepared with the aid of reconstituted membrane complexes containing membrane lipids. Reconstituted membrane complexes assembled from [14C]monoglucosyl diglyceride and delipidated membrane proteins gave optimal complement fixation titers before saturation of the complexes with the ]14C]monoglucosyl diglyceride. The phosphoglycolipid of the membrane, GPDD, was anticomplementary as a pure lipid, a cholesterol liposome, and a reconstituted membrane complex. This anticomplementary activity, which was caused by 3 mug of pure GPDD, affected both human and guinea pig comp...Continue Reading

References

Jun 1, 1974·Infection and Immunity·N Romano, G Scarlata
Oct 1, 1974·Journal of Bacteriology·H G SchieferM Krüpe
Apr 1, 1968·The Biochemical Journal·N ShawW L Koostra
Nov 1, 1970·Journal of Bacteriology·J M dos Santos MotaL L van Deenen
Dec 1, 1970·Bacteriological Reviews·N Shaw
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S RazinR M Chanock
Dec 1, 1967·The Journal of Experimental Medicine·M J Polley, H J Müller-Eberhard
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J A HaxbyS C Kinsky
Nov 9, 1970·Biochemical and Biophysical Research Communications·W Fischer
Oct 1, 1968·Journal of Bacteriology·B L Beckman, G E Kenny
Apr 1, 1969·The Australian Journal of Experimental Biology and Medical Science·P PlackettR M Lemcke
Sep 1, 1967·The Biochemical Journal·P Plackett, E J Shaw
Dec 16, 1957·Annals of the New York Academy of Sciences·M M RAPPORT, L GRAF
Jan 1, 1963·The American Journal of Tropical Medicine and Hygiene·J F KENT, E H FIFE
Sep 1, 1965·Journal of Bacteriology·J D PollackR C Cleverdon

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Citations

Apr 18, 1977·Biochimica Et Biophysica Acta·A Wieslander, L Rilfors
Aug 1, 1985·Infection and Immunity·L R WashburnL K Roberts

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