Abstract
Glycolipids, the predominant class of lipids in the membranes of Acholeplasma laidlawii, are the haptenic determinants that react with anti-A. Laidlawii serum to fix complement. The predominant complement-fixing activity of the membrane glycolipids was associated with the monoglucoysyl diglyceride, diglucosyl diglyceride, glycerlphosphoryl diglucosyl diglyceride (GPDD), and an unknown lipid B, which did not react with ninhydrin but release glucose and glycerol and traces of phosphorus upon hydrolysis. The glycolipids monoglucosyl diglyceride and diglucosyl diglyceride or GPDD and unknown lipid B were paired as a result of their cross-reactions with selective antisera prepared with the aid of reconstituted membrane complexes containing membrane lipids. Reconstituted membrane complexes assembled from [14C]monoglucosyl diglyceride and delipidated membrane proteins gave optimal complement fixation titers before saturation of the complexes with the ]14C]monoglucosyl diglyceride. The phosphoglycolipid of the membrane, GPDD, was anticomplementary as a pure lipid, a cholesterol liposome, and a reconstituted membrane complex. This anticomplementary activity, which was caused by 3 mug of pure GPDD, affected both human and guinea pig comp...Continue Reading
References
Jun 1, 1974·Infection and Immunity·N Romano, G Scarlata
Oct 1, 1974·Journal of Bacteriology·H G SchieferM Krüpe
Dec 1, 1974·Infection and Immunity·T SugiyamaW R Mayberry
Apr 1, 1968·The Biochemical Journal·N ShawW L Koostra
Oct 22, 1968·Biochimica Et Biophysica Acta·N Shaw
Nov 1, 1970·Journal of Bacteriology·J M dos Santos MotaL L van Deenen
Feb 1, 1972·Journal of Bacteriology·D F Kiszkiss, R J Downey
Aug 1, 1972·The Biochemical Journal·N ShawH M Verheij
May 1, 1974·Infection and Immunity·A J DeSiervo
Apr 1, 1972·The Biochemical Journal·P ToonJ Baddiley
May 1, 1970·Immunochemistry·M M Mayer
Dec 1, 1970·Bacteriological Reviews·N Shaw
Oct 1, 1970·Proceedings of the National Academy of Sciences of the United States of America·S RazinR M Chanock
Dec 1, 1967·The Journal of Experimental Medicine·M J Polley, H J Müller-Eberhard
Sep 1, 1969·Proceedings of the National Academy of Sciences of the United States of America·J A HaxbyS C Kinsky
Nov 24, 1970·Biochemistry·K Inoue, S C Kinsky
Mar 1, 1970·Journal of General Microbiology·A J Wicken, K W Knox
Nov 9, 1970·Biochemical and Biophysical Research Communications·W Fischer
Jun 22, 1971·Biochemistry·K InoueS C Kinsky
Mar 9, 1971·Biochimica Et Biophysica Acta·R M ColeS Razin
Oct 1, 1968·Journal of Bacteriology·B L Beckman, G E Kenny
Apr 1, 1969·The Australian Journal of Experimental Biology and Medical Science·P PlackettR M Lemcke
Aug 1, 1969·Journal of Bacteriology·P F Smith
Mar 1, 1966·Immunochemistry·R A NelsonN Tamura
Sep 1, 1967·The Biochemical Journal·P Plackett, E J Shaw
Dec 16, 1957·Annals of the New York Academy of Sciences·M M RAPPORT, L GRAF
Jan 1, 1963·The American Journal of Tropical Medicine and Hygiene·J F KENT, E H FIFE
Apr 1, 1970·Infection and Immunity·S RazinR M Chanock
Sep 1, 1965·Journal of Bacteriology·J D PollackR C Cleverdon