PMID: 6398186Jan 1, 1984Paper

Immunological properties of porcine enterotoxigenic Escherichia coli heat-stable (ST) enterotoxins

Comparative Immunology, Microbiology and Infectious Diseases
L Pesti, K Lukács

Abstract

Cross-linking semipurified and purified heat-stable enterotoxin (2023 ST) to carrier proteins with glutaraldehyde pigs and rabbits were immunized. With ST antisera thus raised, a relatively simple, two-step method for isolation of ST is described. The method involved immunoadsorbent column chromatography procedure, and elution of the retained material with 6 M urea at pH 3.0 yielded purified ST with enterotoxin activity, controlled in suckling mouse test. ST purity was checked with a special staining technique in PAGE, anionic-exchange chromatography and immunodiffusion.

References

Sep 29, 1979·The Veterinary Record·A I Adetosoye, M Awad-Masalmel
Apr 1, 1967·The Journal of Pathology and Bacteriology·H W Smith, S Halls

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