Immunological properties of the beta-subunit of human chorionic gonadotropin. I. Effect of chemical and enzymatic modifications

Endocrinology
R D GhaiO P Bahl

Abstract

The beta-subunit of hCG (hCG beta) displays immunological cross-reactivity with human LH (hLH). A detailed study was undertaken to investigate the effect of chemical and enzymatic modifications on the immunological behavior of hCG beta, particularly on its cross-reactivity with hLH, with a view to obtaining a highly hCG-specific antigen. hCG beta was modified in both the carbohydrate and protein parts of the molecule. The carbohydrate part was modified enzymatically by sequential cleavage of monosaccharides by specific glycosidases, and the protein portion was modified chemically in the amino and carboxyl groups and in the cystinyl, tyrosyl, histidyl, and arginyl residues. These derivatives were immunologically evaluated by RIAs; their hCG beta activities were measured in the [125I]hCG beta-anti-hCG beta system, and the hLH cross-reactivity in the [125I]hLH-anti-hLH system. The present studies have led to the following conclusions. 1) The carbohydrate does not play a significant role in the immunological activity of hCG beta. 2) The antigenic determinants of hCG beta reside primarily in the polypeptide chain and are conformational rather than sequential in nature. 3) hCG beta has two types of antigenic determinants, those which...Continue Reading

Citations

Oct 14, 1983·Clinica Chimica Acta; International Journal of Clinical Chemistry·M Soos, K Siddle
Jan 1, 1987·Comparative Biochemistry and Physiology. B, Comparative Biochemistry·P HallinL E Edqvist
Nov 15, 1987·Archives of Biochemistry and Biophysics·H T Sojar, O P Bahl
Dec 1, 1981·Archives of Biochemistry and Biophysics·H M MadnickO P Bahl
Aug 31, 1982·Biochemical and Biophysical Research Communications·N SwaminathanG D Braunstein
Oct 30, 1981·Biochemical and Biophysical Research Communications·N K Kalyan, O P Bahl
Jan 28, 2009·Reproductive Biology and Endocrinology : RB&E·Laurence A Cole

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