Immunological response to renal cryoablation in an in vivo orthotopic renal cell carcinoma murine model

The Journal of Urology
Surena F MatinJames H Finke

Abstract

The immunological consequences of cryoablation for renal cell carcinoma are largely unknown. Cryoablation is an attractive therapeutic option for tumors due to its minimally invasive nature. Cryoablation is also potentially immunogenic. We describe the development of an animal model to deliver in vivo renal cryotherapy to orthotopically implanted renal cell carcinoma and the results of multiple immunological interrogations after cryoablation. Four to 6-week-old female Balb/c mice (Jackson Laboratories, Bar Harbor, Maine) underwent renal subcapsular implantation of the syngeneic murine renal cell carcinoma Renca. Two weeks later contact cryoablation was done in tumor bearing kidneys. Another group of animals underwent cryoablation of normal kidneys. Animals were sacrificed 2 weeks after tumor injection or 1 and 2 weeks after cryoablation, respectively. Kidneys, spleens and draining lymph nodes were harvested. Evaluation consisted of immunohistochemistry, immunofluorescence and gene expression profiling using reverse-transcriptase polymerase chain reaction. Subcapsular tumor implantation was successful in all cases and confirmed histologically. No significant lymphocytic infiltrate was seen in tumor only animals but those treated...Continue Reading

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Citations

Apr 1, 2014·Advanced Drug Delivery Reviews·Johan Unga, Mitsuru Hashida
Aug 9, 2011·Urology·Ithaar H Derweesh
Mar 8, 2011·Surgical Oncology Clinics of North America·Jose A KaramSurena F Matin
Sep 18, 2010·The Journal of Urology·Jose A KaramSurena F Matin
Nov 16, 2010·International Journal of Urology : Official Journal of the Japanese Urological Association·David S FinleyJean B Dekernion
Mar 31, 2015·PloS One·Akira KasuyaYoshiki Tokura
Jan 11, 2011·Technology in Cancer Research & Treatment·M LiY Q Cai
Dec 17, 2014·Korean journal of urology·Hyung Keun KimSeok Ho Kang
May 7, 2014·Cardiovascular and Interventional Radiology·Neeraj B PurohitDavid J Breen
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May 22, 2019·Insights Into Imaging·B M AartsF M Gómez
Feb 25, 2020·Translational Oncology·Paweł SobczukAnna M Czarnecka

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