Immunological responses and actin dynamics in macrophages are controlled by N-cofilin but are independent from ADF.

PloS One
Friederike JönssonWalter Witke

Abstract

Dynamic changes in the actin cytoskeleton are essential for immune cell function and a number of immune deficiencies have been linked to mutations, which disturb the actin cytoskeleton. In macrophages and dendritic cells, actin remodelling is critical for motility, phagocytosis and antigen presentation, however the actin binding proteins, which control antigen presentation have been poorly characterized. Here we dissect the specific roles of the family of ADF/cofilin F-actin depolymerizing factors in macrophages and in local immune responses. Macrophage migration, cell polarization and antigen presentation to T-cells require n-cofilin mediated F-actin remodelling. Using a conditional mouse model, we show that n-cofilin also controls MHC class II-dependent antigen presentation. Other cellular processes such as phagocytosis and antigen processing were found to be independent of n-cofilin. Our data identify n-cofilin as a novel regulator of antigen presentation, while ADF on the other hand is dispensable for macrophage motility and antigen presentation.

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Citations

Dec 17, 2015·Translational Stroke Research·Qasim AlhadidiZahoor A Shah
Jul 23, 2015·Journal of Proteomics·Kees MeijerHan Roelofsen
Oct 17, 2014·Immunological Reviews·Spencer A Freeman, Sergio Grinstein
May 21, 2015·Brain Structure & Function·Ria UhlemannGolo Kronenberg
Mar 22, 2014·The Journal of Immunology : Official Journal of the American Association of Immunologists·Yan XuStephan Grabbe
Jan 28, 2014·The Plant Cell·Jessica L Henty-RidillaChristopher J Staiger

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Methods Mentioned

BETA
FACS
PECs
electronmicroscopy
FCS
transfection
transfector
Assay
transgenic
ELISA
scanning electron microscopy

Software Mentioned

FlowJo
Treestar
MetaMorph
Axiovision
StarStation

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