Immunological role of thiol-dependent peroxiredoxin gene in Macrobrachium rosenbergii

Fish & Shellfish Immunology
Jesu ArockiarajSubha Bhassu

Abstract

In this study, we have reported a full length of peroxiredoxin (designated MrPrdx) gene, identified from the transcriptome of freshwater prawn Macrobrachium rosenbergii. The complete gene sequence of the MrPrdx is 940 base pairs in length, and encodes 186 amino acids. MrPrdx contains a long thioredoxin domain in the amino acid sequence between 34 and 186. The gene expressions of MrPrdx in healthy and the infectious hypodermal and hematopoietic necrosis virus (IHHNV) challenged M. rosenbergii were examined using quantitative real time polymerase chain reaction. MrPrdx is highly expressed in all the other tissues of M. rosenbergii considered for analysis and the highest in gills. The expression is strongly up-regulated in gills after IHHNV infection. To understand MrPrdx functional properties, the recombinant MrPrdx protein was expressed in Escherichia coli BL21 (DE3) and purified. A peroxidise activity assay was conducted using recombinant MrPrdx protein at different concentrations. This peroxidises activity showed that the recombinant MrPrdx is a thiol-dependant protein. Additionally, this result showed that recombinant MrPrdx protein, as a secretory protein can remove H₂O₂ and protect DNA damage. This finding leads a possible ...Continue Reading

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Citations

Dec 4, 2015·Fish & Shellfish Immunology·Mukesh Kumar ChaurasiaJesu Arockiaraj
Jun 10, 2015·Fish & Shellfish Immunology·Jesu ArockiarajNaif Abdullah Al-Dhabi
Nov 16, 2013·Molecular Immunology·Prasanth BhattJesu Arockiaraj
Aug 6, 2013·Molecular Immunology·Abirami ArasuJesu Arockiaraj
Aug 28, 2021·Developmental and Comparative Immunology·Xing-Chao RenQing-Hui Liu

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