Immunological studies in diabetic rat recipients with a pancreatic islet cell allograft in the brain

Transplantation
W J Tze, J Tai

Abstract

Intracerebrally (IC) transplanted outbred Wistar and inbred Lewis (AgB1/1) strain rat islets and pancreatic endocrine cells (PEC) were able to function for a prolonged period in nonimmunosuppressed diabetic inbred ACI (AgB4/4) rats across a major histocompatibility barrier. All recipients were sensitized to various degrees to the donor antigens, as demonstrated by circulating cytotoxic antibody, irrespective of the survival of the IC graft. Nevertheless, the antidonor antibody titers in the IC islet and PEC graft recipients were lower and peaked later when compared with ACI recipients that received an intraportal islet allograft. PEC were also transplanted IC in immunized ACI recipients. In recipients hyperimmunized by repeated splenocyte injections, accelerated PEC graft rejection was observed. In recipients with weaker immunization by intraportal whole islet allograft 2 months prior to the IC allograft, the IC PEC allografts were also rejected. To assess if ACI rats with long-term-functioning IC islet/PEC allograft developed tolerance to the donor antigens, these animals were transplanted with a donor-strain skin graft. The skin grafts were all rejected in a first-set fashion similar to normal control ACI rats. Also, 7/12 and...Continue Reading

Citations

Aug 21, 2013·Current Diabetes Reports·Sophie VériterDenis Dufrane
Jan 1, 1992·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·S J Sullivan
Feb 25, 2015·Diabetes/metabolism Research and Reviews·Daniel LazardKonstantin Bloch
Jan 28, 2017·Journal of Tissue Engineering and Regenerative Medicine·Konstantin BlochAbraham Weizman
Oct 20, 2017·Tissue Engineering. Part B, Reviews·Haitao ZhuGuoqiang Chen

❮ Previous
Next ❯

Related Concepts

Related Feeds

Allogenic & Autologous Therapies

Allogenic therapies are generated in large batches from unrelated donor tissues such as bone marrow. In contrast, autologous therapies are manufactures as a single lot from the patient being treated. Here is the latest research on allogenic and autologous therapies.