Apr 30, 1936

IMMUNOLOGICAL STUDIES WITH THE VIRUS OF INFECTIOUS LARYNGOTRACHEITIS OF FOWLS USING THE DEVELOPING EGG TECHNIQUE

The Journal of Experimental Medicine
F M Burnet

Abstract

(1) The technique of chorioallantoic membrane inoculation has been applied to the study of the virus of infectious laryngotracheitis as it occurs amongst Australian poultry. (2) When suitably diluted suspensions of virus are inoculated, isolated foci or pocks appear whose macroscopic form and histological structure is characteristic. The numbers of these foci may be used as a measure of the amount of virus present. (3) Two distinct types of focus are produced by laryngotracheitis strains, one being characteristic of epizootic strains from New South Wales and from America, the other of a Victorian strain which is of very low virulence for fowls. (4) No qualitative antigenic differences can be detected amongst these strains but the epizootic strains are more readily neutralised by immune serum than the enzootic Victorian strain. (5) A study of the inactivation of the virus by immune serum shows that (a) the process of inactivation requires time for its completion in vitro; (b) the proportionate reduction in titre produced by a given concentration of antiserum is independent of the initial virus concentration; (c) reactivation by dilution is readily demonstrable; (d) virus in the presence of small concentrations of immune serum pr...Continue Reading

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References

Mentioned in this Paper

Laryngotracheitis
Bacteriophages
Immune Sera
Transcription Initiation
Virulence
Dilution Technique
Enzootic
Sprains and Strains
Chorioallantoic Membrane
In Vitro [Publication Type]

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