PMID: 3611Jan 1, 1976

Immunology of DNA. I. The influence of reaction conditions on the Farr assay as used for the detection of anti-ds DNA

Journal of Immunological Methods
L A AardenT E Feltkamp

Abstract

The sensitivity and the specificity of the Farr assay for the detection of antibodies to double stranded (ds) DNA depends very much on the reaction conditions. The interaction between ds DNA and anti-ds DNA is inhibited when ionic strength and pH are increased. ds DNA is bound by normal sera at ionic strength lower than 0.11 M NaCl and at physiological ionic strength when the pH is lower than 7.2. Substantial binding of DNA by normal serum takes place in barbitone, borate or Tris-HCl buffers at concentrations of 30 mM or higher, even at a pH higher than 7.2. Such binding is due to Clq and is only partially prevented by heating the serum for 30 min at 56 degrees C, but 10 mM phosphate in the incubation mixture completely prevents it. Standardization of ionic strength, pH, phosphate concentration, incubation volume and DNA-serum ratio enhances the diagnostic usefulness of the Farr assay.

References

Jan 9, 1971·Lancet·M W Greaves, A Skillen
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Citations

Nov 1, 1978·Arthritis and Rheumatism·R L RubinR I Carr
Nov 1, 1990·Journal of Clinical Immunology·A RuffattiS Todesco
Nov 1, 1992·Molecular Biology Reports·R Smeenk, M Hylkema
Mar 1, 1990·Clinical Rheumatology·R J SmeenkT Swaak
Apr 13, 2013·Immunologic Research·D S Pisetsky
Jan 1, 1977·Journal of Immunological Methods·L A AardenE R de Groot
May 10, 1979·Journal of Immunological Methods·E V Barnett
Jan 1, 1981·Journal of Immunological Methods·P T Strickland, J M Boyle
Dec 30, 1982·Journal of Immunological Methods·R J SmeenkL A Aarden
Jun 24, 1992·Journal of Immunological Methods·A J Pesce, J G Michael
Feb 1, 1990·Clinical and Experimental Immunology·S AotsukaR Yokohari
Jan 1, 1978·Australian and New Zealand Journal of Medicine·J Webb
Jul 1, 1995·European Journal of Neurology : the Official Journal of the European Federation of Neurological Societies·G P AnzolaA Bonetti
Feb 1, 1981·Annals of the Rheumatic Diseases·A J SwaakT E Feltkamp
Oct 1, 1990·Annals of the Rheumatic Diseases·R H DerksenR J Smeenk
May 1, 1982·Journal of Clinical Pathology·R P StokesR A Thompson
Apr 22, 1988·Journal of Immunological Methods·R J SmeenkT Swaak
Jun 7, 2003·Scandinavian Journal of Immunology·L BjörkmanD S Pisetsky
Jan 1, 1982·International Journal of Nuclear Medicine and Biology·H F Graafland, A L Wagner
Jul 24, 2002·Clinics in Laboratory Medicine·David F Keren
Jan 1, 1987·Scandinavian Journal of Rheumatology·R H DerksenL Kater
Nov 1, 1985·Scandinavian Journal of Immunology·P FaaberJ H Berden
Aug 11, 2006·Lupus·A M Rouquette, C Desgruelles
Dec 1, 1979·Arthritis and Rheumatism·C R Steinman
Sep 1, 1991·Clinical and Experimental Immunology·T H WinklerJ R Kalden
Jan 1, 1980·Immunological Communications·E R de GrootC J van Oss
Mar 1, 1979·Arthritis and Rheumatism·A J SwaakT E Feltkamp

Related Concepts

Binding Sites, Antibody
Ampholytes
DNA, Double-Stranded
Hydrogen-Ion Concentration
Lupus Erythematosus, Systemic
Osmolality
Orthophosphate
Radioimmunoassay
Sodium Chloride, (24)NaCl

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