Immunomagnetic detection and clinical significance of micrometastatic tumor cells in malignant melanoma patients

Clinical Cancer Research : an Official Journal of the American Association for Cancer Research
Ragnar S FayeOystein Fodstad

Abstract

Positive associations between the presence of micrometastatic tumor cells and disease aggressiveness have been reported in several tumor types, but the clinical implications are still not established. We wanted to test a new, sensitive immunomagnetic detection method on bone marrow (BM) and peripheral blood (PB) samples from patients with malignant melanoma and relate the findings to clinical outcome. Samples from 210 patients admitted for relapse of cutaneous melanoma were examined. Mononuclear cell fractions isolated from BM and PB were incubated with superparamagnetic particles coated with antimelanoma antibodies. Live tumor cells with bound beads were isolated with a magnet and identified in a microscope as cell-bead rosettes. Beads without antibody or with an irrelevant antibody were used as controls. The whole procedure was completed within 2-3 h. The identity of the cells was confirmed with a new double labeling procedure with fluorescent microparticles. Rosetted melanoma cells were found in BM aspirates of 35 of 186 (19%) patients, but in only 2 of 208 (1%) PB samples. The controls were all negative. After a median observation time of 1.1 year (range, 0-6.8 years), patients with tumor cells in BM showed a significantly ...Continue Reading

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Citations

Sep 18, 2012·Journal of Translational Medicine·James B FreemanMelanie Ziman
Oct 20, 2012·Journal of Colloid and Interface Science·Ludmila C FidaleThomas Heinze
Nov 26, 2010·Cancer Treatment Reviews·Andrianos NezosMichael Koutsilieris
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Mar 14, 2008·Melanoma Research·Ragnar S FayeSteinar Aamdal
Aug 15, 2013·British Journal of Cancer·A K RudK Boye

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