Immunomagnetic-electrochemiluminescent detection of Escherichia coli O157 and Salmonella typhimurium in foods and environmental water samples.

Applied and Environmental Microbiology
H Yu, J G Bruno

Abstract

Hemorrhagic Escherichia coli O157:H7 strains and other virulent enteric pathogens can pose a serious health threat in tainted meats, poultry, and even drinking water. Traditional culture-based methods for assay of enteric pathogens in foods and water sources are relatively slow, and results can be ambiguous. Immunomagnetic separation (IMS) and detection methods have been investigated and appear promising for rapid bacterial assay of foods and environmental samples. In this work, a commercial sensor which combines IMS with electrochemiluminescence (ECL) detection is evaluated for detection of E. coli O157 and Salmonella typhimurium in foods and fomites. Results indicate that detection limits are in the range of 100 to 1,000 bacteria per ml in pristine buffer for E. coli O157 and S. typhimurium, respectively, or 1,000 to 2,000 bacteria per ml in food samples (depending on the sample) and that total processing and assay time is rapid (< 1 h) even in food samples. An immunologic "hook" or high-antigen-concentration prozone effect was observed above 10(4) and 10(5) bacteria per ml for E. coli O157 and S. typhimurium, respectively. IMS was accomplished in milk, juices, serum, supernatant fluids from ground beef, finely minced chicken...Continue Reading

References

Mar 1, 1991·Journal of Immunological Methods·J M Luk, A A Lindberg
Nov 1, 1990·Applied and Environmental Microbiology·E SkjerveO Olsvik
Jul 1, 1989·Journal of Clinical Microbiology·S B March, S Ratnam
Oct 4, 1988·Journal of Immunological Methods·M Stenberg, H Nygren
Dec 1, 1988·Journal of Clinical Microbiology·A LundF Vartdal
Oct 1, 1987·Applied and Environmental Microbiology·M P Doyle, J L Schoeni
Mar 24, 1983·The New England Journal of Medicine·L W RileyM L Cohen
Jul 1, 1995·Journal of Bioluminescence and Chemiluminescence·H YuD L Gatto-Menking
Feb 1, 1991·Applied and Environmental Microbiology·J A MorganJ R Saunders
Mar 1, 1992·Journal of Food Protection·Anita J G OkrendCharles P Lattuada
Jan 1, 1995·Journal of Food Protection·Stephen D WeagantKaren G Jinneman

❮ Previous
Next ❯

Citations

May 2, 2002·Transfusion Medicine·J Rider, A Newton
Jun 30, 2005·Analytical and Bioanalytical Chemistry·Ismail H BoyaciWilliam R Heineman
Sep 13, 2006·Analytical Sciences : the International Journal of the Japan Society for Analytical Chemistry·Vepa K RaoKrishnamurthi Sekhar
Apr 10, 2010·Journal of Food Protection·Dawn M Hunter, Daniel V Lim
Dec 8, 2005·Applied and Environmental Microbiology·Munehiro KubotaKengo Sakaguchi
Jun 18, 2016·Health Security·Norman A DoggettSegaran Pillai
Apr 16, 2017·Applied and Environmental Microbiology·Jenna M DenyesMartin J Loessner
Apr 20, 2001·Critical Reviews in Biotechnology·S ParkR A Durst
Feb 14, 2003·Biotechnology and Bioengineering·Ramaiah Sachidanandham, Karina Yew-Hoong Gin
Oct 20, 2004·Critical Reviews in Microbiology·Karine LemarchandRoland Brousseau
Jan 9, 2004·Applied and Environmental Microbiology·Chris Rowe TaittFrances S Ligler
Jun 13, 2014·Lab on a Chip·Amir Sanati Nezhad
Jun 27, 2001·Applied and Environmental Microbiology·D R Shelton, J S Karns
Oct 21, 2000·Journal of Food Protection·L J RobertsonA T Campbell
Aug 1, 2009·Bioanalysis·Paul W RhyneRussell S Weiner
May 1, 2004·Critical Reviews in Microbiology·Kelly A Stevens, Lee-Ann Jaykus
Oct 15, 2005·Clinical Microbiology Reviews·Daniel V LimMarianne F Kramer
Oct 27, 2017·Current Medicinal Chemistry·Agnieszka ChylewskaMariusz Makowski
Apr 2, 1999·Applied and Environmental Microbiology·L GoodridgeM Griffiths
Jun 10, 2004·Chemical Reviews·Mark M Richter
Sep 28, 2001·Biochemical and Biophysical Research Communications·J G BrunoC A Batt
Jun 9, 1998·Journal of Immunological Methods·J D Brewster, R S Mazenko

❮ Previous
Next ❯

Related Concepts

Related Feeds

Anxiety Disorders

Discover the latest research on anxiety disorders including agoraphobia, panic disorder, obsessive-compulsive disorder, and post-traumatic stress disorder here.