Immunomics of the koala (Phascolarctos cinereus)

Immunogenetics
Kendra C AbtsJ Andrew DeWoody

Abstract

The study of the koala transcriptome has the potential to advance our understanding of its immunome--immunological reaction of a given host to foreign antigens--and to help combat infectious diseases (e.g., chlamydiosis) that impede ongoing conservation efforts. We used Illumina sequencing of cDNA to characterize genes expressed in two different koala tissues of immunological importance, blood and spleen. We generated nearly 600 million raw sequence reads, and about 285 million of these were subsequently assembled and condensed into ~70,000 subcomponents that represent putative transcripts. We annotated ~16% of these subcomponents and identified those related to infection and the immune response, including Toll-like receptors (TLRs), RIG-I-like receptors (RLRs), major histocompatibility complex (MHC) genes, and koala retrovirus (KoRV). Using phylogenetic analyses, we identified 29 koala genes in these target categories and report their concordance with currently accepted gene groups. By mapping multiple sequencing reads to transcripts, we identified 56 putative SNPs in genes of interest. The distribution of these SNPs indicates that MHC genes (34 SNPs) are more diverse than KoRV (12 SNPs), TLRs (8 SNPs), or RLRs (2 SNPs). Our s...Continue Reading

Associated Datasets

Mar 4, 2015·Jamie A. IvyJ. Andrew DeWoody

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Citations

Nov 25, 2016·Journal of Virology·K J ChappellP R Young
Jul 3, 2017·Immunogenetics·Yuanyuan ChengKatherine Belov
Aug 30, 2018·PeerJ·Parice A BrandiesKatherine Belov
Oct 4, 2020·Retrovirology·HaoQiang ZhengWilliam M Switzer
Feb 6, 2021·Immunogenetics·Emma PeelKatherine Belov
Sep 9, 2021·Proceedings of the National Academy of Sciences of the United States of America·Briony A JoyceKeith J Chappell

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