Immunomodulator mediated changes in plasma membrane calcium ATPase in controlling visceral leishmaniasis

Experimental Parasitology
Nivedita RoySubrata Majumdar

Abstract

Immunomodulation is an emerging concept to combat infection in recent years. Immunomodulators like arabinosylated-lipoarabinomannan (Ara-LAM) and glycyrrhizic-acid (GA) possess anti-leishmanial property, whereas sodium-antimony-gluconate (SAG) is still considered as the first choice for chemotherapy against leishmaniasis. During infection, invasion of Leishmania donovani needs the potential requirement of Ca2+, which is further responsible for apoptosis in intracellular amastigotes. However, suppression of elevated intracellular calcium by the activation of plasma-membrane-calcium-ATPase (PMCA4) facilitates survival of L. donovani in the host. In the present study, SAG, Ara-LAM, and GA were found to evoke significant increase in intracellular Ca2+ in L. donovani infected macrophages by inhibiting PMCA4. Moreover, PMCA4 inhibition by TFP or PMCA4 siRNA elevated the level of PKCβ, whereas calcium-independent upregulation of PKCζ remained unchanged in infected macrophages. Furthermore, application of immunomodulators in infected macrophages resulted in down-regulation of PKCζ, conversion of anti-inflammatory to pro-inflammatory cytokines and inhibition of PMCA4. Plasma membrane-associated ceramide which is known to be elevated dur...Continue Reading

References

Nov 1, 1989·Trends in Neurosciences·K P Huang
Jun 18, 1982·Science·A B Clarkson, B O Amole
Feb 24, 2001·Molecular and Cellular Biochemistry·S BhattacharyyaS Majumdar
Oct 5, 2006·Current Medicinal Chemistry·Manar M Salem, Karl A Werbovetz
May 29, 2008·Apoptosis : an International Journal on Programmed Cell Death·Rakhee DasHemanta K Majumder
Nov 28, 2008·The Journal of Antimicrobial Chemotherapy·Surajit BhattacharjeeSubrata Majumdar
Jul 28, 2009·Annual Review of Medicine·David H Broide
Apr 21, 2010·International Journal of Antimicrobial Agents·Rupashree SenMitali Chatterjee
Nov 18, 2011·Molecular Biology International·Arun Kumar HaldarSyamal Roy
Apr 21, 2012·The Journal of Infectious Diseases·Saikat MajumderSubrata Majumdar
May 17, 2012·The Journal of Antimicrobial Chemotherapy·Surajit BhattacharjeeSubrata Majumdar
Feb 5, 2013·Trends in Parasitology·Nicola BakerDavid Horn
Jul 30, 2016·Pharmacological Research : the Official Journal of the Italian Pharmacological Society·Naeem K PatilEdward R Sherwood
Aug 31, 2016·Current Topics in Medicinal Chemistry·Jonathan M FuraMarcos M Pires
Aug 23, 2017·Scientific Reports·Mark KorthalsUlrich Thomas
Apr 29, 2018·Biochimica Et Biophysica Acta. Molecular Cell Research·Ilari PulliKid Törnquist
Jan 9, 2019·Frontiers in Immunology·Hanne LocySarah K Maenhout

❮ Previous
Next ❯

Citations

May 4, 2021·Frontiers in Microbiology·Julio FloresLeslie Chavez-Galan

❮ Previous
Next ❯

Related Concepts

Related Feeds

Apoptosis

Apoptosis is a specific process that leads to programmed cell death through the activation of an evolutionary conserved intracellular pathway leading to pathognomic cellular changes distinct from cellular necrosis