Immunomodulatory action of the DNA methyltransferase inhibitor SGI-110 in epithelial ovarian cancer cells and xenografts

Epigenetics : Official Journal of the DNA Methylation Society
Pragya SrivastavaElizabeth A Griffiths

Abstract

We aimed to determine the effect of SGI-110 on methylation and expression of the cancer testis antigens (CTAs) NY-ESO-1 and MAGE-A in epithelial ovarian cancer (EOC) cells in vitro and in vivo and to establish the impact of SGI-110 on expression of major histocompatibility (MHC) class I and Intracellular Adhesion Molecule 1 (ICAM-1) on EOC cells, and on recognition of EOC cells by NY-ESO-1-specific CD8+ T-cells. We also tested the impact of combined SGI-110 and NY-ESO-1-specific CD8+ T-cells on tumor growth and/or murine survival in a xenograft setting. EOC cells were treated with SGI-110 in vitro at various concentrations and as tumor xenografts with 3 distinct dose schedules. Effects on global methylation (using LINE-1), NY-ESO-1 and MAGE-A methylation, mRNA, and protein expression were determined and compared to controls. SGI-110 treated EOC cells were evaluated for expression of immune-modulatory genes using flow cytometry, and were co-cultured with NY-ESO-1 specific T-cell clones to determine immune recognition. In vivo administration of SGI-110 and CD8+ T-cells was performed to determine anti-tumor effects on EOC xenografts. SGI-110 treatment induced hypomethylation and CTA gene expression in a dose dependent manner both ...Continue Reading

References

Feb 1, 1990·European Journal of Cancer : Official Journal for European Organization for Research and Treatment of Cancer (EORTC) [and] European Association for Cancer Research (EACR)·C SessaF Cavalli
Sep 3, 1996·Proceedings of the National Academy of Sciences of the United States of America·J G HermanS B Baylin
Nov 9, 2002·Bioinformatics·Long-Cheng Li, Rajvir Dahiya
Jan 18, 2003·International Journal of Cancer. Journal International Du Cancer·Brad StoneBrad H Nelson
Jul 11, 2003·Proceedings of the National Academy of Sciences of the United States of America·Sacha GnjaticLloyd J Old
Oct 29, 2003·Journal of Immunological Methods·Michael R BettsRichard A Koup
Mar 9, 2005·Journal of Clinical Oncology : Official Journal of the American Society of Clinical Oncology·Wolfram E SamlowskiAdam R Karpf
Dec 14, 2005·Proceedings of the National Academy of Sciences of the United States of America·Eiichi SatoKunle Odunsi
May 12, 2006·Molecular Cancer Research : MCR·Frank WischnewskiHeidi Schwarzenbach
Oct 6, 2006·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·David S SchrumpSeth M Steinberg
Jul 10, 2007·Cancer Research·Christine B YooPeter A Jones
Jul 27, 2007·Proceedings of the National Academy of Sciences of the United States of America·Kunle OdunsiSacha Gnjatic
Sep 6, 2007·Epigenetics : Official Journal of the DNA Methylation Society·Adam R Karpf
Feb 2, 2008·International Journal of Cancer. Journal International Du Cancer·Atsushi NatsumeJun Yoshida
Apr 22, 2008·International Journal of Cancer. Journal International Du Cancer·Carlo Stresemann, Frank Lyko
Jun 4, 2008·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anna Woloszynska-ReadAdam R Karpf
Sep 4, 2009·International Journal of Cancer. Journal International Du Cancer·Julia KarbachElke Jäger
Feb 8, 2011·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Anna Woloszynska-ReadAdam R Karpf
Mar 29, 2012·Proceedings of the National Academy of Sciences of the United States of America·Kunle OdunsiElke Jäger
May 3, 2012·Cancer Research·Daniela MateiKenneth P Nephew
Sep 8, 2012·Advances in Experimental Medicine and Biology·Elizabeth A Griffiths, Steven D Gore
Oct 4, 2012·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Paul SabbatiniSacha Gnjatic
Nov 10, 2012·Cancer Immunology, Immunotherapy : CII·Sandra CoralMichele Maio
Jul 11, 2013·Epigenetics : Official Journal of the DNA Methylation Society·Smitha R JamesAdam R Karpf
Feb 19, 2014·Cancer Immunology Research·Kunle OdunsiAdam R Karpf
Oct 16, 2014·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Fang FangKenneth P Nephew

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Citations

Jul 15, 2015·Molecular Oncology·Yuting KuangNouri Neamati
Oct 18, 2015·Trends in Cardiovascular Medicine·Hui TaoJun Li
May 10, 2015·Pharmacology & Therapeutics·Kristen M Meldi, Maria E Figueroa
Sep 21, 2016·Epigenetics : Official Journal of the DNA Methylation Society·Simone JueligerManlio Vinciguerra
Sep 22, 2016·Clinical Epigenetics·Inês GraçaCarmen Jerónimo
Jun 5, 2019·Current Topics in Medicinal Chemistry·Yong-Tao DuanYongfang Yao
Jul 11, 2020·Cancer Immunology, Immunotherapy : CII·Aaraby Y NielsenMorten F Gjerstorff
Feb 13, 2018·Oncology Letters·Zhong-Tian BaiWen-Ce Zhou
Feb 16, 2019·Journal of Experimental & Clinical Cancer Research : CR·Boran PangShouping Xu
Feb 6, 2019·Therapeutic Advances in Hematology·Jan Philipp BewersdorfAmer M Zeidan
May 1, 2018·Frontiers in Pharmacology·Ângela Marques-MagalhãesCarmen Jerónimo
Mar 20, 2019·Expert Review of Anticancer Therapy·Jan Philipp BewersdorfAmer M Zeidan
Nov 5, 2019·Frontiers in Genetics·Marcelo L RibeiroGaël Roué
Jun 27, 2019·Epigenetics : Official Journal of the DNA Methylation Society·Ashley R MaiuriHeather M O'Hagan
Oct 19, 2020·BMC Cancer·Isamu HoshinoHideaki Shimada
Mar 19, 2016·Cancer Research·Katherine B ChiappinelliStephen B Baylin
Sep 28, 2017·Clinical Cancer Research : an Official Journal of the American Association for Cancer Research·Elizabeth A GriffithsMichael J Nemeth
Nov 27, 2020·Therapeutic Advances in Hematology·Jan Philipp BewersdorfThomas Prebet
May 29, 2020·Seminars in Cancer Biology·Kah Keng Wong
Apr 1, 2021·Molecular Cancer Therapeutics·Weini WangKenneth P Nephew
Aug 19, 2021·Cancer Cell International·Weiwei XieXipeng Wang
Aug 17, 2021·Nutrition Research Reviews·Tatiana MontoyaCatalina Alarcón-de-la-Lastra

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Methods Mentioned

BETA
xenografts
MDS
xenograft
flow cytometry
PCR

Software Mentioned

GraphPad Prism

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