Immunomodulatory and cytotoxic activities of euphol.

Life Sciences
Thais L de OliveiraFlávio Luís Beltrame

Abstract

This study evaluated the effect of euphol isolated from Euphorbia umbellata (Pax) Bruyns latex on the activation of complement pathways (classical (CP), alternative (AP) and lectin (LP)), neutrophil chemotaxis, cytotoxic activity, cell morphology and death in HRT-18 and 3T3 cells lines. CP and AP were assessed using hemolytic assays and ELISA for LP; neutrophil chemotaxis was performed using Boyden's chamber; cytotoxicity was evaluated by neutral red methodology and characteristics of cell death were assessed by cell morphology with hematological staining. Although euphol increased CP activation (38% at a concentration of 976.1 μM), an inhibitory effect on AP, LP (31% and 32% reduction in the concentration of 976.1 μM) and neutrophil chemotaxis (inhibit 84% of neutrophil migration at a concentration 292.9 μM) was observed. In addiction euphol was able to induce significant cell death in a time-dependent manner, presenting an IC50 of 70.8 μM and 39.2 μM for HRT-18 and 3T3 cell lines respectively and it was also observed apoptotic characteristics as cellular rounding, chromatin condensation and blebs formation for both cell lines. Euphol has a potential use for the treatment of complement-related inflammatory diseases due to its ...Continue Reading

References

Mar 28, 2001·Annals of the New York Academy of Sciences·T J RogersJ J Oppenheim
Sep 17, 2004·Scandinavian Journal of Gastroenterology·H YttingH J Nielsen
Apr 19, 2008·Journal of Cellular and Molecular Medicine·Morten Harboe, Tom Eirik Mollnes
Jul 8, 2008·Planta medica·Jeanine Marie NardinAlmeriane Maria Weffort-Santos
Sep 23, 2011·Pharmaceutical Biology·Girija KuttanRamadasan Kuttan
Dec 14, 2011·Biochemical Pharmacology·Rafael Cypriano DutraJoão B Calixto
May 29, 2012·Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association·Ming-Wei LinYaw-Bin Huang
May 28, 2013·Seminars in Immunology·Ruben PioJohn D Lambris
Sep 7, 2013·Journal of Ethnopharmacology·Thais Latansio de OliveiraFlávio Luís Beltrame
Jan 1, 2014·Journal of Natural Products·Wei-Hua SongDao-Feng Chen
Apr 2, 2014·Planta medica·Antonio C M MunhozFlávio L Beltrame
Feb 24, 2015·Molecular Immunology·Joost P M MelisPaul W H I Parren
Dec 10, 2015·Mediators of Inflammation·Zvi Granot, Jadwiga Jablonska
Dec 22, 2015·Immunobiology·Srinivas MamidiMichael Kirschfink
Feb 26, 2016·Journal of Ethnopharmacology·Lívia Eidam Camargo LuzFlávio Luís Beltrame
May 24, 2016·Nature Reviews. Nephrology·Daniel RicklinJohn D Lambris
Jun 16, 2016·Journal of Ethnopharmacology·Bruno Rodrigo MinozzoFlávio Luis Beltrame
Mar 2, 2017·The Journal of Clinical Investigation·Vahid Afshar-Kharghan
Sep 19, 2017·Chemico-biological Interactions·Keyu He, Meng Tang
Sep 19, 2017·Nature Reviews. Immunology·Edimara S ReisJohn D Lambris
Sep 1, 2018·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Luiza S CruzFlávio L Beltrame
Oct 28, 2019·Chemistry & Biodiversity·Lorena BaviaIara Jose Messias-Reason
Sep 9, 2020·Journal of Ethnopharmacology·Thais Latansio de OliveiraIara Jose Messias-Reason

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