Immunomodulatory and cytotoxic effects of Nigella sativa and thymoquinone on rat splenocytes

Food and Chemical Toxicology : an International Journal Published for the British Industrial Biological Research Association
Zahra GholamnezhadMohammad Hossein Boskabady

Abstract

Three different concentrations of Nigella sativa (N. sativa) ethanolic extract, thymoquinone (TQ), dexamethasone, and saline were examined to see whether they had any effects on cell viability, proliferation, and interleukin 4 (IL-4) and interferon-γ (IFN-γ) secretion in non-stimulated, phytohemagglutinin (PHA) and concavaline A (Con A)-stimulated splenocytes. In PHA and Con A-stimulated splenocytes, cell viability and proliferation were increased and Con A shifted cytokine profile towards Th2 balance. Dexamethasone treatment showed a suppression in viability, IFNγ and IL-4 secretion in non-stimulated and stimulated splenocytes. Extract and TQ reduced the viability and inhibited the proliferation of stimulated and non-stimulated splenocytes concentration-dependently. Higher concentrations of N. sativa (1000 mg/ml) and TQ (5 and 10 mg/ml) reduced the secretion of IL-4 in stimulated cells. Two higher concentrations of N. sativa had decreased IFNγ secretion in both stimulated and non-stimulated cells. In non-stimulated cells, only the highest and in Con A-stimulated cells, all TQ concentrations had inhibited IFNγ secretion. The highest concentration of N. sativa increased IFNγ/IL-4 ratio in both stimulated and non-stimulated cells...Continue Reading

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Citations

Apr 11, 2017·Journal of Pharmacopuncture·Mahdieh Jafari ShahroudiHossein Hosseinzadeh
May 30, 2019·Drug and Chemical Toxicology·Zahra GholamnezhadMahmoud Hosseini
May 11, 2018·Oxidative Medicine and Cellular Longevity·Md JakariaDong-Kug Choi
Jul 1, 2017·Frontiers in Pharmacology·A G M MostofaMuhammad Shahdaat Bin Sayeed
Oct 10, 2020·Journal of Cancer Prevention·Rohini GomathinayagamDanny N Dhanasekaran
Aug 18, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Thamara Figueiredo ProcópioThiago Henrique Napoleão
Jun 2, 2021·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Mohammad Reza KhazdairMohammad Hossein Boskabady
Jun 11, 2021·Pharmaceutical Biology·Mohammad Reza KhazdairMahmood Sadeghi
Aug 13, 2021·Environmental Science and Pollution Research International·Mohammad Reza Khazdair, Mohammad Hossein Boskabady

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