Immunomodulatory cross-talk between conjunctival goblet cells and dendritic cells

PloS One
Laura Contreras-Ruiz, Sharmila Masli

Abstract

Goblet cells are secretory epithelial cells of mucosal tissues that confer protection from environmental agents or pathogens via expression and secretion of soluble mucins. Loss of these cells is associated with several chronic inflammatory disorders of the mucosa. Although demonstrated to transfer antigens from the luminal surface to stromal cells in the intestinal mucosa, it is not known if goblet cells contribute to the regulation of an immune response. In this study we report that similar to intestinal and respiratory mucosal epithelia, mouse ocular surface epithelia predominantly express the TGF-ß2 isoform. Specifically, we demonstrate the ability of goblet cells to express TGF-ß2 and increase it in response to Toll-Like Receptor 4 mediated stimulus in cultures. Goblet cells not only express TGF-ß2, but are also able to activate it in a thrombospondin-1 (TSP-1) dependent manner via their cell surface receptor CD36. Furthermore, goblet cell derived soluble factors that possibly include TGF-ß2, alter dendritic cell (DC) phenotype to a tolerogenic type by downregulating DC expression of MHC class II and co-stimulatory molecules CD80, CD86 and CD40. Thus our study demonstrates goblet cells as a cellular source of active TGF-ß2...Continue Reading

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Citations

Jun 13, 2015·Eye & Contact Lens·Laura García-PosadasYolanda Diebold
Jul 15, 2015·Journal of Ocular Pharmacology and Therapeutics : the Official Journal of the Association for Ocular Pharmacology and Therapeutics·Laura Soriano-RomaníYolanda Diebold
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Mar 11, 2017·International Ophthalmology Clinics·Cintia S de Paiva
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Datasets Mentioned

BETA
GM-CSF

Methods Mentioned

BETA
PCR
flow cytometry
fluorescence microscopy

Software Mentioned

FlowJo

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