Immunomodulatory Effects of CP-25 on Splenic T Cells of Rats with Adjuvant Arthritis

Inflammation
Yang WangWei Wei

Abstract

Rheumatoid arthritis (RA) is an autoimmune disease in which T cells play an important role. Paeoniflorin-6-oxy-benzenesulfonate (CP-25) shows a strong anti-inflammatory and immunomodulatory effect in the joint of adjuvant arthritis (AA) rats, but the role of the spleen function is still unclear. The aim of this study was to research how CP-25 regulated spleen function of AA rats. Male Sprague-Dawley rats were administered with CP-25 (50 mg/kg) orally from day 17 to 29 after immunization. The spleen histopathological changes were analyzed by hematoxylin-eosin staining. G protein-coupled receptor kinases (GRKs) and prostaglandin receptor subtypes (EPs) were screened by Western blot and immunohistochemistry. The co-expression of GRK2 and EP2 as well as GRK2 and EP4 was measured by immunofluorescence and co-immunoprecipitation. The expression of GRK2 and EP4 in splenic T cells was further detected by immunofluorescence. CP-25 was found to relieve the secondary paw swelling, attenuate histopathologic changes, and downregulate GRK2, EP2 and EP4 expression in AA rats. Additionally, CP-25 not only downregulated the co-expression of GRK2 and EP4 but also downregulated GRK2, EP4 expression in splenic T cells of AA rats. From these result...Continue Reading

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Citations

Aug 17, 2020·Critical Care : the Official Journal of the Critical Care Forum·Joseph MillerSudarshan Hebbar
Nov 6, 2020·The Journal of Immunology : Official Journal of the American Association of Immunologists·Xinming WangWei Wei
Apr 4, 2021·Medicina·Xiangyu ZhaoYoung-Ok Son
Oct 31, 2018·Biomedicine & Pharmacotherapy = Biomédecine & Pharmacothérapie·Yi-Jin WuWei Wei

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Methods Mentioned

BETA
Co-immunoprecipitation
confocal scanning microscopy
Flow
flow cytometry
immunoprecipitation
co-IP

Software Mentioned

GraphPad Prism
Pro Plus
ImageJ Launcher Symmetry
SPSS
GraphPad
Image

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