Immunomodulatory effects of four polysaccharides purified from Erythronium sibiricum bulb on macrophages.

Glycoconjugate Journal
Chunli ChenXue Li

Abstract

Four neutral polysaccharides (ESBP1-1, ESBP1-2, ESBP2-1 and ESBP3-1) were successfully purified from the water extracted crude polysaccharides of Erythronium sibiricum bulbs through the combination of DEAE Sepharose CL-6B and Sephadex G-100 chromatography; their average molecular weights were 1.3 × 104, 1.7 × 104, 9.4 × 105 and 4.1 × 105 Da, respectively. Monosaccharide component analysis indicated that ESBP1-1 and ESBP1-2 were mainly composed of glucose (Glc). ESBP2-1 was composed of Glc, galactose (Gal) and arabinose, with a molar ratio of 24.3:1.1:1, whereas ESBP3-1 comprised Glc and Gal at a molar ratio of 14.8:1. In-vitro study showed that all of the four polysaccharides were able to considerably promote the proliferation and neutral red phagocytosis of RAW 264.7 macrophage cell. They could also stimulate the production of the cell lines' secretory molecules [nitric oxide, tumour necrosis factor-α (TNF-α) and interleukin-1β (IL-1β)] in a dose-dependent manner. However, ESBP1-2 was not included in IL-1β. Overall, these results suggested that polysaccharides from E. sibiricum bulbs can be developed as immunomodulatory ingredients for complementary medicines or functional foods. However, further animal or clinical studies are...Continue Reading

References

Aug 1, 1973·Analytical Biochemistry·N Blumenkrantz, G Asboe-Hansen
Jan 1, 1996·Journal of Clinical Laboratory Analysis·J Ni, D Hollander
May 26, 2005·International Immunopharmacology·Kun Yeong Lee, Young Jin Jeon
Jan 24, 2006·International Immunopharmacology·Igor A Schepetkin, Mark T Quinn
Sep 11, 2008·Genesis : the Journal of Genetics and Development·Dmitry A Ovchinnikov
Apr 19, 2012·Phytotherapy Research : PTR·Qiang YuMing-Yong Xie
Aug 12, 2014·International Journal of Biological Macromolecules·Jiao DouYurong Guo
Oct 16, 2014·International Journal of Biological Macromolecules·Yi-Qun DuJun-Hui Wang
Jan 18, 2015·International Journal of Biological Macromolecules·Chao LiLi-Jun You
Jun 10, 2015·International Journal of Biological Macromolecules·Lijun WangGuixing Ren
Oct 16, 2015·Critical Reviews in Food Science and Nutrition·Jian-Hua XieMing-Yong Xie
Nov 4, 2015·International Journal of Biological Macromolecules·Chunli ChenWenhuan Ding
Jan 16, 2016·Biochemical and Biophysical Research Communications·Su-Yan DongYun-Cheng Wu
May 2, 2016·International Journal of Biological Macromolecules·Qiu-Lian LuoXuan Luo
Jul 3, 2016·International Journal of Biological Macromolecules·Ming-Ming LiuLian-Gen Shi
Jul 23, 2016·Phytomedicine : International Journal of Phytotherapy and Phytopharmacology·Sensen LinShengtao Yuan
Jul 18, 2017·International Journal of Biological Macromolecules·Rena KasimuXue Li
Jul 8, 2019·International Journal of Biological Macromolecules·Ying-Jie ZengWen-Yong Lou
Jan 21, 2020·International Journal of Biological Macromolecules·Tingting ZhengJiangping Fan
Aug 30, 2020·International Journal of Biological Macromolecules·Hongyan LiXihong Yang
Jan 14, 2021·Carbohydrate Polymers·Yunxia YangJianfa Zhang

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