Immunomodulatory effects of heat stress and lipopolysaccharide on the bursal transcriptome in two distinct chicken lines

BMC Genomics
Melissa S MonsonSusan J Lamont

Abstract

Exposure to heat stress suppresses poultry immune responses, which can increase susceptibility to infectious diseases and, thereby, intensify the negative effects of heat on poultry welfare and performance. Identifying genes and pathways that are affected by high temperatures, especially heat-induced changes in immune responses, could provide targets to improve disease resistance in chickens. This study utilized RNA-sequencing (RNA-seq) to investigate transcriptome responses in the bursa of Fabricius, a primary immune tissue, after exposure to acute heat stress and/or subcutaneous immune stimulation with lipopolysaccharide (LPS) in a 2 × 2 factorial design: Thermoneutral + Saline, Heat + Saline, Thermoneutral + LPS and Heat + LPS. All treatments were investigated in two chicken lines: a relatively heat- and disease-resistant Fayoumi line and a more susceptible broiler line. Differential expression analysis determined that Heat + Saline had limited impact on gene expression (N = 1 or 63 genes) in broiler or Fayoumi bursa. However, Thermoneutral + LPS and Heat + LPS generated many expression changes in Fayoumi bursa (N = 368 and 804 genes). Thermoneutral + LPS was predicted to increase immune-related cell signaling and cell migra...Continue Reading

References

Jul 1, 1994·Poultry Science·E Paramithiotis, M J Ratcliffe
Jan 1, 1993·European Journal of Immunology·E Paramithiotis, M J Ratcliffe
Mar 16, 2000·Developmental and Comparative Immunology·W I MuirA J Husband
Feb 16, 2002·Methods : a Companion to Methods in Enzymology·K J Livak, T D Schmittgen
Mar 12, 2003·Oncogene·Ya-Wei QiangStuart Rudikoff
Dec 6, 2005·The Journal of Biological Chemistry·Yanjing XiaoGuolong Zhang
Jul 29, 2006·Arteriosclerosis, Thrombosis, and Vascular Biology·Peter I BontaCarlie J M de Vries
Sep 11, 2007·Proceedings of the National Academy of Sciences of the United States of America·Ryo GoitsukaMax D Cooper
Jul 12, 2008·Nature Reviews. Immunology·Frank J T StaalMachteld M Tiemessen
Jul 1, 1979·Avian Pathology : Journal of the W.V.P.A·E D HellerM Perek
Jun 10, 2009·Bioinformatics·Heng LiUNKNOWN 1000 Genome Project Data Processing Subgroup
Oct 30, 2009·The Journal of Immunology : Official Journal of the American Association of Immunologists·Zhi-Qin WangZe-Guang Han
Apr 13, 2011·Stem Cells and Development·Marijke W MaijenburgCarlijn Voermans
May 3, 2011·Veterinary Microbiology·A van DijkH P Haagsman
Jun 8, 2011·Veterinary Immunology and Immunopathology·Phongsakorn ChuammitriDušan Palić
Feb 22, 2012·Proceedings of the National Academy of Sciences of the United States of America·Marlys S FassettChristophe Benoist
Apr 21, 2012·Animal Science Journal = Nihon Chikusan Gakkaihō·Daichi IjiriMiho Hirabayashi
Aug 21, 2012·Avian Pathology : Journal of the W.V.P.A·W M Quinteiro-FilhoJ Palermo-Neto
Oct 30, 2012·Bioinformatics·Alexander DobinThomas R Gingeras
Jan 22, 2013·Nature Immunology·Takashi SekiyaAkihiko Yoshimura
Apr 4, 2014·Bioinformatics·Anthony M BolgerBjoern Usadel
Sep 28, 2014·Bioinformatics·Simon AndersWolfgang Huber
Dec 18, 2014·Genome Biology·Michael I LoveSimon Anders
Apr 29, 2015·The Journal of Steroid Biochemistry and Molecular Biology·Stephen SafeRonald B Tjalkens
Jul 8, 2015·The Journal of Immunology : Official Journal of the American Association of Immunologists·Daniel CreanEvelyn P Murphy
Sep 4, 2015·Journal of Animal Physiology and Animal Nutrition·J Tang, Z Chen

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Citations

Mar 25, 2020·Journal of Animal Science·Marcos H Rostagno
Jun 27, 2019·Poultry Science·Nikhil K NuthalapatiGregory T Pharr
Apr 14, 2021·Cell Stress & Chaperones·Haitham G Abo-Al-ElaRasha A Al Wakeel

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Methods Mentioned

BETA
RNA-seq
PCR
GTPases
chip
Assay

Software Mentioned

FastX
HTSeq
Ingenuity Pathway Analysis ( IPA
Fluidigm Real - Time PCR Analysis
DESeq2
Fluidigm
IPA
samtools
Biomark
Trimmomatic

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