Immunomodulatory effects of Viscum album agglutinin-I on natural immunity

Anti-cancer Drugs
T HajtoR Saller

Abstract

In 24 h cultured human peripheral blood mononuclear cells, treated with various (1 microgram/ml to 1 ng/ml) concentrations of Viscum album agglutinin-I, quantitative assessment of DNA breaks labelled with terminal deoxynucleotidyl transferase revealed a dose-dependent Viscum album agglutinin-I-induced apoptosis above a lectin concentration of 10 ng/ml. After 24 h incubation of peripheral blood mononuclear cells with non-cytotoxic concentrations of Viscum album agglutinin-I (10 and 1 ng/ml), messenger (m)RNA expression and secretion of a panel of cytokines were evaluated by reverse polymerase chain reaction and by enzyme-linked immunosorbent assay (ELISA), respectively. The lectin induced expression of interleukin-1 alpha, interleukin-1 beta, interleukin-6, tumor necrosis factor-alpha, interferon-gamma, granulocyte-monocyte colony stimulating factor and interleukin-10 genes, but no expression of interleukin-2 or interferon-gamma production could be detected. In addition, cellular components of the natural immune system (such as monocytes and granulocytes) bound Viscum album agglutinin-I molecules to a higher degree than lymphocytes. To establish the modulatory potency of Viscum album agglutinin-I on the natural immunity of human...Continue Reading

Citations

Oct 17, 2007·Molekuliarnaia biologiia·O Iu SudarkinaIu V Kozlov
Aug 20, 2014·BioMed Research International·Mohsen MarvibaigiSaravana Kumar Jaganathan
Jan 28, 2009·Clinical and Experimental Hypertension : CHE·Mirjana RadenkovicLjiljana Gvozdenovic
Aug 29, 2017·BioMed Research International·C M LopesR Oliveira
Apr 29, 2004·Journal of Peptide Science : an Official Publication of the European Peptide Society·Roland WackerWolfgang Voelter

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