Immunomorphology and molecular biology of mixed primary liver cancers: is Nestin a marker of intermediate-cell carcinoma?

Histopathology
Deborah MalviFrancesco Vasuri

Abstract

Primary mixed liver cancers (PLCs), combined hepatocellular-cholangiocellular (cHCC-CC) and intermediate-cell carcinomas are rare tumours characterised by different molecular mechanisms. Nestin is a marker of progenitor cells with a promising application in human tumours. The aims of the present paper are (i) to determine the expression of Nestin in mixed PLCs; and (ii) to correlate the PLC immunoprofile with the gene expression in each tumour component. We selected 28 mixed PLCs, 13 (46.4%) cHCC-CC and 15 (53.6%) intermediate-cell carcinomas. The immunohistochemistry panel consisted of keratin 7, keratin 19, CD56 and Nestin. Next-generation sequencing analysis was performed on 17 cases (27 specimens) using a multi-gene custom panel. The differentiated HCC and CC components of cHCC-CC were negative for Nestin in all cases. The intermediate areas of cHCC-CC were immunoreactive for Nestin in 92.3% of cases, for CD56 in 76.9% and for K7/K19 in all cases. The immunoprofile of the intermediate-cell carcinomas showed 73.3% of cases positive for Nestin and 66.7% for CD56. TP53 and TERT were the most frequently mutated genes (31.3% and 17.6% of samples, respectively). Mutations were also found in IDH1, IDH2, PIK3CA and NRAS genes. Inte...Continue Reading

References

Sep 21, 2002·Molecular and Cellular Biology·Eva González-SuárezMaría A Blasco
Jan 20, 2004·The Journal of Comparative Neurology·John L MignoneGrigori Enikolopov
Aug 4, 2004·Journal of Hepatology·Dominique Cazals-HatemJessica Zucman-Rossi
Aug 30, 2005·Developmental Dynamics : an Official Publication of the American Association of Anatomists·Anatoli S GleibermanGrigori Enikolopov
Mar 27, 2013·Proceedings of the National Academy of Sciences of the United States of America·Patrick J KillelaHai Yan
Mar 28, 2013·Current Neurology and Neuroscience Reports·Adam L CohenHoward Colman
Dec 9, 2014·Cell Cycle·Supriya K SahaNabeel Bardeesy
Aug 6, 2015·Journal of Clinical Pathology·Francesco VasuriAntonia D'Errico-Grigioni
Aug 11, 2015·Nature Genetics·Hiromi NakamuraTatsuhiro Shibata
May 18, 2016·World Journal of Gastroenterology : WJG·An-Qiang WangHai-Tao Zhao
Jul 15, 2016·Journal of Experimental & Clinical Cancer Research : CR·Yan ZhangHong Shen
Jan 4, 2017·Gastroenterology·Daniela SiaJosep M Llovet
Jun 24, 2017·Digestive Diseases and Sciences·Silvia FittipaldiAntonia D'Errico
Jul 22, 2017·Modern Pathology : an Official Journal of the United States and Canadian Academy of Pathology, Inc·Naoki OishiRyohei Katoh
Aug 31, 2017·Acta Neuropathologica Communications·Yujin LeeSung-Hye Park
Jan 24, 2018·Hepatology : Official Journal of the American Association for the Study of Liver Diseases·Elizabeth BruntNeil Theise
Nov 8, 2018·Endocrine Connections·Dario de BiaseAlessia Ciarrocchi
Nov 20, 2018·La Presse médicale·Chiara VillaAnna Luisa Di Stefano

❮ Previous
Next ❯

Citations

Feb 6, 2021·Journal of Hepatology·Aurélie BeaufrèreValérie Paradis
Mar 7, 2021·Trends in Cancer·Valentina LeoneMathias Heikenwalder
Apr 4, 2021·Diagnostics·Mario Pérez-SayánsJosé M Suárez-Peñaranda
Aug 24, 2021·Clinical Medicine Insights. Oncology·Anna Szymańska-ChabowskaMariusz Chabowski

❮ Previous
Next ❯

Related Concepts

Trending Feeds

COVID-19

Coronaviruses encompass a large family of viruses that cause the common cold as well as more serious diseases, such as the ongoing outbreak of coronavirus disease 2019 (COVID-19; formally known as 2019-nCoV). Coronaviruses can spread from animals to humans; symptoms include fever, cough, shortness of breath, and breathing difficulties; in more severe cases, infection can lead to death. This feed covers recent research on COVID-19.

Blastomycosis

Blastomycosis fungal infections spread through inhaling Blastomyces dermatitidis spores. Discover the latest research on blastomycosis fungal infections here.

Nuclear Pore Complex in ALS/FTD

Alterations in nucleocytoplasmic transport, controlled by the nuclear pore complex, may be involved in the pathomechanism underlying multiple neurodegenerative diseases including Amyotrophic Lateral Sclerosis and Frontotemporal Dementia. Here is the latest research on the nuclear pore complex in ALS and FTD.

Applications of Molecular Barcoding

The concept of molecular barcoding is that each original DNA or RNA molecule is attached to a unique sequence barcode. Sequence reads having different barcodes represent different original molecules, while sequence reads having the same barcode are results of PCR duplication from one original molecule. Discover the latest research on molecular barcoding here.

Chronic Fatigue Syndrome

Chronic fatigue syndrome is a disease characterized by unexplained disabling fatigue; the pathology of which is incompletely understood. Discover the latest research on chronic fatigue syndrome here.

Evolution of Pluripotency

Pluripotency refers to the ability of a cell to develop into three primary germ cell layers of the embryo. This feed focuses on the mechanisms that underlie the evolution of pluripotency. Here is the latest research.

Position Effect Variegation

Position Effect Variagation occurs when a gene is inactivated due to its positioning near heterochromatic regions within a chromosome. Discover the latest research on Position Effect Variagation here.

STING Receptor Agonists

Stimulator of IFN genes (STING) are a group of transmembrane proteins that are involved in the induction of type I interferon that is important in the innate immune response. The stimulation of STING has been an active area of research in the treatment of cancer and infectious diseases. Here is the latest research on STING receptor agonists.

Microbicide

Microbicides are products that can be applied to vaginal or rectal mucosal surfaces with the goal of preventing, or at least significantly reducing, the transmission of sexually transmitted infections. Here is the latest research on microbicides.