PMID: 7019309Apr 1, 1981Paper

Immunoperoxidase localization of a glycoprotein on plasma membrane of secretory epithelium from human breast

The Journal of Histochemistry and Cytochemistry : Official Journal of the Histochemistry Society
A Imam, Z A Tökés

Abstract

A glycoprotein component of human milk-fat globule membrane, which is said to derive form apical plasma membrane of mammary secretory epithelium, has been purified. The purified glycoprotein yields a single band under reduced condition and has an estimated molecular weight of 70,000 daltons on sodium dodecyl sulfate polyacrylamide gel electrophoresis. The glycoprotein was termed "secretory epithelial membrane antigen" (SEMA-70) to indicate its origin and molecular size. Specific antisera to SEMA-70 were raised. Using the peroxidase-antiperoxidase method of staining, the presence of SEMA-70 was demonstrated on the apical plasma membrane of the mammary epithelial cells lining the ducts of both normal and lactating breast. No significant staining of cytoplasm was observed. these observations add further evidence as to the origin of milk-fat globule membrane. Although the antigen continues to be expressed on breast adenocarcinoma cells, its cellular distribution appears to be different. The potential usefulness of specific antisera to SEMA-70 is suggested to identify rearrangements in cellular architecture due to pathological changes.

Citations

Jan 1, 1984·Virchows Archiv. A, Pathological Anatomy and Histopathology·R Mariani-CostantiniF Rilke
Mar 1, 1985·Cancer Genetics and Cytogenetics·S R WolmanA J Hackett
Jan 1, 1984·Biochimica Et Biophysica Acta·H S SmithA J Hackett
Sep 18, 1998·Journal of Cerebral Blood Flow and Metabolism : Official Journal of the International Society of Cerebral Blood Flow and Metabolism·H IshiiK Mori
Oct 2, 2007·Journal of Stroke and Cerebrovascular Diseases : the Official Journal of National Stroke Association·Kolammal NageswariIwao Kanno
Jan 1, 1983·Annals of the New York Academy of Sciences·C R Taylor
Jan 1, 1983·Annals of the New York Academy of Sciences·R D CardiffJ Schlom
Aug 15, 1984·International Journal of Cancer. Journal International Du Cancer·J HilkensM van der Valk

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